Abstract
Monocytes, macrophages, neutrophils, and eosinophils are able to generate and release reactive oxygen species. The reactive oxygen species are generated by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase which is activated by a number of different soluble and particulate agents. This activation results in the reduction of molecular oxygen to the potentially toxic oxygen species superoxide anion (O2 −), or hydrogen peroxide (H2O2), with NADPH serving as the electron donor. In the presence of eosinophil peroxidase, H2O2 may then give rise to the potentially cytotoxic hypohalous acids such as HOBr. The increase in oxygen consumption is termed the respiratory burst (1,2).
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References
Lundqvist H., Follin P., Khalfan L., and Dahlgren C. (1996) Phorbol myristate acetate—induces NADPH oxidase activity in human neutrophils: only half the story has been told. J. Leukoc. Biol. 59, 270–279.
Remick D. G. and Villarete L. (1996) Regulation of cytokine gene expression by reative oxygen and reactive nitrogen intermediates. J. Leukoc. Biol. 59, 471–475.
Lambeth J. D. (1988) Activation of the respiratory burst oxidase in neutrophils: on the role of membrane-derived second messaengers, Ca2+, and protein kinase. C. J. Bioenerg. Biomemb. 20, 709–733.
Bieber T., de la Salle H., Wollenberg A., Hakimi J., Chizzonite R., Ring J., Hanau D., and de la Salle C. (1993) Human epidermal Langerhans cells express the high affinity receptor for immunoglobulin E (Fc epsilon RI). J. Exp. Med. 175, 1285–1290.
Busse W. W. and Sedgwick J. B. (1992) Eosinophils in asthma. Ann. Allergy 286, 286–290.
Gleich G. J., Adolphoson C. R., and Leifermann K. M. (1993) The biology of the eosinophilic eosinophilic leukocyte. Annu. Rev. Med. 44, 85–101.
Romagnani S. (1997) The Th1/Th2 paradigm. Immunol. Today 18, 263–266.
Coyle A. J., Ackermann S., Burch R., Proud D., and Irvin C. G. (1995) Human eosinophil granule major basic protein and synthetic polycations induce airway hyperresponsiveness In vivo dependent on bradykinin generation. J. Clin. Invest. 95, 1735–1740.
Elsner J., Dichmann S., and Kapp A. (1995) Activation of the respiratory burst in human eosinophils by chemotaxins requires intracellular calcium fluxes. J. Invest. Dermatol. 105, 231–236.
Kapp A. (1993) The role of eosinophils in the pathogenesis of atopic dermatitis—eosinophil granule proteins as markers of disease activity. Allergy 48, 1–5.
Rochester C. L., Ackermann S. J., Zheng T., and Elias J. A. (1996) Eosinophil-fibroblast interactions—granule major basic protein interacts with IL-1 and transforming growth factor-beta in the stimulation of lung fibroblast IL-6-type cytokine production. J. Immunol. 156, 4449–4456.
Rosenberg H. F. and Tiffany H. L. (1994) Characterization of the eosinophil granule proteins recognized by the activation-specific antibody EG2. J. Leukoc. Biol. 56, 502–506.
Elsner J., Hochstetter R., Kimmig D., and Kapp A. (1996) Human eotaxin represents a potent activator of the respiratory burst of human eosinophils. Eur. J. Immunol. 26, 1919–1925.
Elsner J., Oppermann M., Czech W., Dobos G., Schöpf E., Norgauer J., and Kapp A. (1994) C3a activates reactive oxygen radical species production and intracellular calcium transients in human eosinophils. Eur. J. Immunol. 24, 518–522.
Kapp A., Zeck Kapp G., Czech W., and Schöpf E. (1994) The chemokine RANTES is more than a chemoattractant: Characterization of its effect on human eosinophil oxidative metabolism and morphology in comparison with IL-5 and GM-CSF. J. Invest. Dermatol. 102, 906–914.
Zeck Kapp G., Kroegel C., Riede U. N., and Kapp A. (1995) Mechanisms of human eosinophil activation by complement protein C5a and platelet-activating factor: similar functional responses are accompanied by different morphologic alterations. Allergy 50, 34–47.
Kernen P., Wymann M. P., von Tscharner V., Deranleau D. A., Tai P. C., Spry C. J., Dahinden C. A., and Baggiolini M. (1991) Shape changes, exocytosis, and cytosolic free calcium changes in stimulated eosinophils. J. Clin. Invest. 87, 2012–2017.
Nagata M., Sedgwick J. B., Bates M. E., Kita H., and Busse W. W. (1995) Eosinophil adhesion to vascular cell adhesion molecule-1 activates superoxide anion generation. J. Immunol. 155, 2194–2202.
Thelen M., Wymann M. P., and Langen H. (1994) Wortmannin binds specifically to 1-phosphatidylinositol 3-kinase while inhibiting guanine nucleotide-binding protein-coupled receptor signaling in neutrophil leukocytes. Proc. Natl. Acad. Sci. USA 91, 4960–4964.
Elsner J., Oppermann M., Czech W., and Kapp A. (1994) C3a activates the respiratory burst in human polymorphonuclear neutrophilic leukocytes via pertussis toxin-sensitive G-proteins. Blood 83, 3324–3331.
Maly F. E., Urwyler A., Rolli H. P., Dahinden C., and de Weck A. L. (1988) A single-photon imaging system for the simultaneous quantitation of luminescent emissions from multiple samples. Anal. Biochem. 168, 462–469.
Wymann M. P., Kernen P., Deranleau D. A., and Baggiolini M. (1989) Respiratory burst oscillations in human neutrophils ad their correlation with fluctuations in apparent cell shape. J. Biol. Chem. 264, 15,829–15,834.
Kapp A., Zeck Kapp G., Danner M., and Luger T. A. (1988) Human granulo-cyte-macrophage colony stimulating factor: An effective direct activator of human polymorphonuclear neutrophilic granulocytes. J. Invest. Dermatol. 91, 49–55.
Elsner J., Oppermann M., and Kapp A. (1996) Detection of C5a receptors on human eosinophils and inhibition of eosinophil effector functions by anti-C5a receptor (CD88) antibodies. Eur. J. Immunol. 26, 1560–1564.
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Elsner, J., Kapp, A. (2000). Reactive Oxygen Release. In: Proudfoot, A.E.I., Wells, T.N.C., Power, C.A. (eds) Chemokine Protocols. Methods in Molecular Biology, vol 138. Humana Press. https://doi.org/10.1385/1-59259-058-6:153
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DOI: https://doi.org/10.1385/1-59259-058-6:153
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