Abstract
Precursors of most secreted and cell surface molecules carry signal sequences at their amino termini. The method coined as signal sequence trap (1–3) takes advantage of the presence of N-terminal signal sequences in most precursor forms of secretory proteins and transmembrane proteins, which are necessary for the proper orientation of the N-terminal of mature forms inside endoplasmic reticulum and exocytotic vesicles. This method enables to selectively clone cDNA species encoding intercellular signal-transducing molecules without biologic assays. In this chapter, an efficient signal sequence trap method based on an Epstein-Barr virus shuttle vector is described (2).
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© 2000 Humana Press Inc.
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Imai, T. (2000). Cloning of Novel Chemokines Using a Signal Sequence Trap Method. In: Proudfoot, A.E.I., Wells, T.N.C., Power, C.A. (eds) Chemokine Protocols. Methods in Molecular Biology, vol 138. Humana Press. https://doi.org/10.1385/1-59259-058-6:11
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DOI: https://doi.org/10.1385/1-59259-058-6:11
Publisher Name: Humana Press
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