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Stress Response pp 217–255Cite as

Analysis of the Mammalian Heat-Shock Response

Inducible Gene Expression and Heat-Shock Factor Activity

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 99))

Abstract

The evolutionarily conserved heat-shock response has been extensively studied as a model for transcriptional regulation. In eukaryotic cells, the regulation of heat-shock gene expression is mediated by a family of related proteins, the heat-shock transcription factors (HSFs) (1–8). Smaller eukaryotes such as yeast and Drosophila melanogaster usually express single members of the HSF family (1–3), while larger eukaryotes express multiple HSFs. At least four HSF family members have been identified in vertebrate systems (4–8). Multiple HSFs may have arisen to allow expression of heat shock proteins under different conditions, such that divergent signaling pathways converge to result in the production of a common class of proteins, the heat-shock proteins.

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Authors and Affiliations

  1. Rice Institute for Biomedical Research, Northwestern University, Evanston, IL

    Anu Mathew, Yanhong Shi, Caroline Jolly & Richard I. Morimoto

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  1. Anu Mathew
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  2. Yanhong Shi
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  3. Caroline Jolly
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  4. Richard I. Morimoto
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  1. ICRF Laboratories, University of Dundee, UK

    Stephen M. Keyse

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© 2000 Humana Press Inc., Totowa, NJ

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Mathew, A., Shi, Y., Jolly, C., Morimoto, R.I. (2000). Analysis of the Mammalian Heat-Shock Response. In: Walker, J.M., Keyse, S.M. (eds) Stress Response. Methods in Molecular Biology™, vol 99. Humana Press. https://doi.org/10.1385/1-59259-054-3:217

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  • DOI: https://doi.org/10.1385/1-59259-054-3:217

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