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T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 145)

Abstract

The superantigens (SAgs) staphylococcal enterotoxins (SEs) are produced by certain strains of Staphylococcus aureus and comprise structurally related bacterial proteins, which are among the most potent mitogens known for murine and human T lymphocytes (1,2). T-cell activation induced by SEs involves binding to constant parts of major histocompatibility complex (MHC) class II molecules and subsequent interaction with T cells expressing certain T-cell receptor (TCR) Vβ chains (3,4). The binding of SAgs to MHC class II molecules does not require processing and structural mutations, and biochemical experiments have demonstrated that the amino acids involved are distant from the antigen binding groove.

Keywords

Major Histocompatibility Complex Class Effector Cell Staphylococcal Enterotoxin Background Release Cell Dissociation Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  1. 1.AstraZenecaLundSweden
  2. 2.Active Biotech,Lund,Sweden,and Department of Medical MicrobiologyUniversity Hospital MASMalmoeSweden

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