Abstract
This chapter defines the terms and examines the configurations used for most applications of ELISA. Such a chapter is important because the possibilities inherent in the systems of ELISA must be understood in order to maximize their versatility in assay design. All heterogeneous systems have three basic parameters:
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1.
One reactant is attached to a solid phase, usually a plastic microtiter plate with an 8 × 12-well format.
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Separation of bound and free reagents, which are added subsequently to the solid phase-attached substance, is by a simple washing step.
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3.
Results are obtained through the development of color.
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© 2001 Humana Press Inc.
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Crowther, J.R. (2001). Systems in ELISA. In: The Elisa Guidebook. Methods in Molecular Biology™, vol 149. Humana Press. https://doi.org/10.1385/1-59259-049-7:9
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DOI: https://doi.org/10.1385/1-59259-049-7:9
Publisher Name: Humana Press
Print ISBN: 978-0-89603-950-6
Online ISBN: 978-1-59259-049-0
eBook Packages: Springer Protocols