Abstract
Radiolabeling methods have been introduced into the study of the biology of mucin for several reasons (1–3). In many instances, the biochemical analysis of mucins in any form may be limited owing to the small amounts of mucosal tissue available, of cells from culture systems, and the difficulty in obtaining normal material for comparison (3–6). The use of radiolabeling in direct assessment of the biochemistry of the metabolism of mucins, in particular their biosynthesis, is well suited to these techniques in the same way they have been adopted for other proteins and glycoconjugates. It is currently of special interest in evaluating the different stages in the maturation, aggregation, and secretion of mucin.
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Corfield, A.P., Myerscough, N., Van Jan-Willem Klinken, B., Einerhand, A.W.C., Dekker, J. (2000). Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin. In: Corfield, A.P. (eds) Glycoprotein Methods and Protocols. Methods in Molecular Biology™, vol 125. Humana Press. https://doi.org/10.1385/1-59259-048-9:227
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DOI: https://doi.org/10.1385/1-59259-048-9:227
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