Abstract
The interaction between killer cells and target cells can be dissected into three steps. First, the two cells form random conjugates mediated by nonspecific interactions between adhesion molecules (1). Second, specific recognition results in the activation of signal transduction pathways coupled to activating receptors and accessory molecules (2). This leads to the third step consisting of perforin and/or fas-ligand mediated delivery of the lethal hit (3). Lethal hit delivery requires a short time; as a consequence killer cells can recycle and kill multiple targets (4).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Springer T. A. (1990) Adhesion receptors of the immune system. Nature 346, 425–434.
Weiss A. and Littman D. R. (1994) Signal transduction by lymphocyte antigen receptors. Cell 76, 263–274.
Henkart P. A. (1994) Lymphocyte-mediated cytotoxicity: two pathways and multiple effector molecules. Immunity 1, 343–346.
Kupfer A. and Singer S. J. (1989) Cell biology of cytotoxic and helper T cell functions: immunofluorescence microscopic studies of single cells and cell couples. Annu. Rev. Immunol. 7, 309–337.
Gray L. S., Gnarra J. R, Sullivan J. A., Mandell G. L., and Engelhard V. H., (1988) Spatial and temporal characteristic of the increase in intracellular Ca+2 induced in cytotoxic lymphocytes by cellular antigen. J. Immunol. 141, 2424–2430.
Grynkiewicz G., Poenie M., and Tsien R. Y. (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440–3450.
Novak E. J. and Rabinovitch P. S. (1994) Improved sensitivity in flow cytometric intracellular ionized calcium measurement using Fluo-3/Fura red fluorescence ratios. Cytometry 17, 135–141.
Campbell K. S., Dessing M., Lopez-Botet M., Cella M., and Colonna M. (1996) Tyrosine phosphorylation of a human killer inhibitory receptor recruits protein tyrosine phosphatase 1C. J. Exp. Med. 184, 93–100.
Dessing M. and McConnell F. (1997) Single-cell calcium release measured on the flow cytometer, in Immunology Methods Manual, Academic Press San Diego, CA, pp. 1267–1272.
Valitutti S., Muller S., Dessing M., and Lanzavecchia A. (1996) Different responses are elicited in cytotoxic T lymphocytes by different levels of T cell receptor occupancy. J Exp Med 183, 1917–1921.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Valitutti, S., Dessing, M. (1999). Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis. In: Campbell, K.S., Colonna, M. (eds) Natural Killer Cell Protocols. Methods in Molecular Biology, vol 121. Humana Press. https://doi.org/10.1385/1-59259-044-6:305
Download citation
DOI: https://doi.org/10.1385/1-59259-044-6:305
Publisher Name: Humana Press
Print ISBN: 978-0-89603-683-3
Online ISBN: 978-1-59259-044-5
eBook Packages: Springer Protocols