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Transfection and Expression of Exogenous Connexins in Mammalian Cells

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Connexin Methods and Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 154))

Abstract

Currently, 15 different connexin genes have been described in the murine genome (14). Different connexins are expressed in a cell specific fashion, and in most cell types two or more connexins have been demonstrated. To characterize defined connexin channels independently of other connexins that are coexpressed in most mammalian cells, exogenous connexin DNAs can be trans-fected and expressed in cultured cells that show a low level of endogenous gap junction channels (5).

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Manthey, D., Willecke, K. (2001). Transfection and Expression of Exogenous Connexins in Mammalian Cells. In: Bruzzone, R., Giaume, C. (eds) Connexin Methods and Protocols. Methods In Molecular Biology™, vol 154. Humana Press. https://doi.org/10.1385/1-59259-043-8:187

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  • DOI: https://doi.org/10.1385/1-59259-043-8:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-658-1

  • Online ISBN: 978-1-59259-043-8

  • eBook Packages: Springer Protocols

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