Skip to main content

Agarose Gel Electrophoresis of Nucleic Acids

  • Protocol

Part of the Springer Protocols Handbooks book series (SPH)

Abstract

DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs (1).

Keywords

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Boffey, S. A. (1984) Isolation of high molecular weight DNA, in Methods in Molecular Biology, vol. 2: Nucleic Acids (Walker, J. M., ed.), Humana, Totowa, NJ, 333–341.

    Google Scholar 

  2. Harrington, R. E. (1993) Studies of DNA bending and flexibility using gel-electrophoresis. Electrophoresis 14, 732–746.

    Article  PubMed  CAS  Google Scholar 

  3. Lane, D., Prentki, P., and Chandler, M. (1992) Use of gel retardation to analyse protein-nucleic acid interactions. Microbiological Reviews 56, 509–528.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Williams, D.R., Rapley, R. (2000). Agarose Gel Electrophoresis of Nucleic Acids. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:67

Download citation

  • DOI: https://doi.org/10.1385/1-59259-038-1:67

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-459-4

  • Online ISBN: 978-1-59259-038-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics