DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs (1).
- Ethidium Bromide
- Sterile Distil Water
- Cellulose Filter Paper
- Polaroid Camera
- Molecular Weight Size
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Boffey, S. A. (1984) Isolation of high molecular weight DNA, in Methods in Molecular Biology, vol. 2: Nucleic Acids (Walker, J. M., ed.), Humana, Totowa, NJ, 333–341.
Harrington, R. E. (1993) Studies of DNA bending and flexibility using gel-electrophoresis. Electrophoresis 14, 732–746.
Lane, D., Prentki, P., and Chandler, M. (1992) Use of gel retardation to analyse protein-nucleic acid interactions. Microbiological Reviews 56, 509–528.
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© 2000 Humana Press Inc., Totowa, NJ
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Williams, D.R., Rapley, R. (2000). Agarose Gel Electrophoresis of Nucleic Acids. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:67
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-459-4
Online ISBN: 978-1-59259-038-4
eBook Packages: Springer Book Archive