Abstract
The high affinity and specificity of the avidin-biotin interaction permit diverse applications in immunology, histochemistry, in situ hybridizations, affinity chromatography, and many other areas (1) (see Chapter 21 and Chapter 22). It was first exploited in immunocytochemical applications in the mid-1970s (2,3), and has since been commonly used to localize antigens in cells and tissues. In this technique, a biotinylated primary or secondary antibody is first applied to the sample, and the detection is accomplished by using labeled avidin. Avidin with a variety of labels are available commercially, including fluorescent, enzyme, iodine, ferritin, or gold labels.
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© 1994 Humana Press Inc.
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Mao, SY. (1994). Biotinylation of Antibodies. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Bilogy, vol 34. Humana Press. https://doi.org/10.1385/0-89603285-X:49
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DOI: https://doi.org/10.1385/0-89603285-X:49
Publisher Name: Humana Press
Print ISBN: 978-0-89603-285-9
Online ISBN: 978-1-59259-521-1
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