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Animal Cell Biotechnology pp 229–238Cite as

Hematopoietic Cells for Cellular and Gene Therapy

II. Expansion Protocols

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Part of the book series: Methods in Biotechnology™ ((MIBT,volume 8))

Abstract

Hematopoietic culture is a rapidly growing area of mammalian cell culture with broad applications. As discussed in Chapter 17, many different types of cells can be produced of multiple lineages and multiple states of differentiation within each lineage. In this chapter, we illustrate the basic techniques and principles of hematopoietic cell culture by providing protocols for hematopoietic cultures of three types: retroviral transduction of CD34+ cells for gene therapy, and expansion of colony-forming unit-granulocyte monocyte (CFU-GM) progenitors and neutrophil postprogenitors for transplantation therapies.

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Author information

Authors and Affiliations

  1. Chemical Engineering Department, Northwestern University, Evanston, IL

    Todd A. McAdams, E. Terry Papoutsakis & William M. Miller

Authors
  1. Todd A. McAdams
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  2. E. Terry Papoutsakis
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  3. William M. Miller
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Editor information

Editors and Affiliations

  1. Bioprocess Research and Development, Eli Lilly & Co., Indianapolis, IN

    Nigel Jenkins

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© 1999 Humana Press Inc., Totowa, NJ

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McAdams, T.A., Papoutsakis, E.T., Miller, W.M. (1999). Hematopoietic Cells for Cellular and Gene Therapy. In: Jenkins, N. (eds) Animal Cell Biotechnology. Methods in Biotechnology™, vol 8. Humana Press. https://doi.org/10.1385/0-89603-547-6:229

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  • DOI: https://doi.org/10.1385/0-89603-547-6:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-547-8

  • Online ISBN: 978-1-59259-486-3

  • eBook Packages: Springer Protocols

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