Skip to main content

Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC

  • Protocol
Hepatitis C Protocols

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 19))

  • 2271 Accesses

Abstract

T-cells are the key players in the field on which the virus and the immune response try to defeat or at least control each other. Two categories of T-cells are involved: CD4+ and CD8+ T-cells. CD4+ and CD8+ T-cells have different characteristics and functions and different roles in the immune response to viruses. This chapter discusses methods to determine the CD4+ T-cell activity.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Suggested Reading

  1. Diepolder, H. M., Jung, M. C., Albert, E., Hoffmann, R. M., Zachoval, R., and Pape, G. R. (1994) HLA and hepatitis B infection [Letter; comment] Lancet 344, 1772,1773.

    ArticleĀ  Google ScholarĀ 

  2. Diepolder, H. M., Zachoval, R., Hoffmann, R. M., Wierenga, E. A., Santantonio, T., Jung, M. C., et al (1995) Possible mechanism involving T-lymphocyte response to non-structural protein 3 in viral clearance in acute hepatitis C virus infection Lancet 346, 1006,1007.

    ArticleĀ  Google ScholarĀ 

  3. Diepolder, H. M., Zachoval, R., Hoffmann, R. M., Jung, M. C., Gerlach, T., and Pape, G. R. (1996) The role of hepatitis C virus specific CD4+ T lymphocytes in acute and chronic hepatitis C. J Mol Med. 74, 583ā€“588.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  4. Diepolder, H. M., Jung, M. C., Wierenga, E., Hoffmann, R. M., Zachoval, R., Gerlach, T. J., et al. (1996) Anergic TH1 clones specific for hepatitis B virus (HBV) core peptides are inhibitory to other HBV core-specific CD4+ T-cells in vitro. J Virol 70, 7540ā€“7548

    PubMedĀ  CASĀ  Google ScholarĀ 

  5. Ferrari, C., Valli, A., Galati, L., Penna, A., Scaccaglia, P., Giuberti, T., et al. (1994) T-cell response to structural and nonstructural hepatitis C virus antigens in persistent and self-limited hepatitis C virus infections. Hepatology 19, 286ā€“295

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  6. Hoffmann, R. M., Diepolder, H. M., Zachoval, R., Zwiebel, F. M., Jung, M. C., Scholz, S., Nitschko, H., Riethmuller, G., and Pape, G. R. (1995) Mapping of immunodominant CD4+ T lymphocyte epitopes of hepatitis C virus antigens and their relevance during the course of chronic infection. Hepatology 21, 632ā€“638.

    PubMedĀ  CASĀ  Google ScholarĀ 

  7. Jung, M. C., Diepolder, H. M., and Pape, G. R. (1994) T-cell recognition of hepatitis B and C viral antigens. Eur. J. Clin Invest 24, 641ā€“650

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  8. Leroux Roels, G., Esquivel, C. A., DeLeys, R., Stuyver, L., Elewaut, A., Philippe, J., et al (1996) Lymphoproliferative responses to hepatitis C virus core, E1, E2, and NS3 in patients with chronic hepatitis C infection treated with interferon alfa. Hepatology 23, 8ā€“16.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  9. Minutello, M. A., Pileri, P., Unutmaz, D., Censini, S., Kuo, G., Houghton, M., et al. (1993) Compartmentalization of T lymphocytes to the site of disease: intrahepatic CD4+ T-cells specific for the protein NS4 of hepatitis C virus in patients with chronic hepatitis C. J. Exp. Med. 178, 17ā€“25.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  10. Missale, G., Bertoni, R., Lamonaca, V., Valli, A., Massari, M., Mori, C, et al. (1996) Different clinical behaviors of acute hepatitis C virus infection are associated with different vigor of the anti-viral cell-mediated immune response. J. Clin. Invest. 98, 706ā€“714.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

Ā© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Gerlach, J.T., Diepolder, H., Pape, G.R. (1998). Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicineā„¢, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:413

Download citation

  • DOI: https://doi.org/10.1385/0-89603-521-2:413

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-521-8

  • Online ISBN: 978-1-59259-260-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics