Abstract
A major limitation of solution phase polymerase chain reaction (PCR) is the inability to visualize and localize amplified product within cellular and tissue specimens. In situ hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic in situ (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (1).
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© 1998 Humana Press Inc., Totowa, NJ
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O’Leary, J. (1998). In Situ Amplification. In: Lo, Y.M.D. (eds) Clinical Applications of PCR. Methods in Molecular Medicine™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-499-2:109
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DOI: https://doi.org/10.1385/0-89603-499-2:109
Publisher Name: Humana Press
Print ISBN: 978-0-89603-499-0
Online ISBN: 978-1-59259-600-3
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