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Arbitrarily Primed PCR Methods for Studying Bacterial Diseases

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Molecular Bacteriology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

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Abstract

The most common application of the polymerase chain reaction (PCR) IS to exponenttally amplify a specific known and predictable sequence from a complex mixture of nucleic acids. This chapter describes a technique that, in contrast, uses PCR with arbitrary primers, to generate a fingerprint of PCR products from complex mixtures of nucleic acids and identify sequence polymorphisms and other differences that distinguish them. This robust and reproducible technique can amplify discrete sets of DNA fragments. When genomic DNAs from different individuals are compared, these differences represent point mutations and variously sized deletions and insertions When different sources of RNA are examined from an isogenic source, differences in the particular set of DNA fragments amplified represent differentially expressed genes.

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Author information

Authors and Affiliations

  1. Urocor Inc., Oklahoma City, OK

    David Ralph

  2. Sidney Kimmel Cancer Center, San Diego, CA

    Michael McClelland

Authors
  1. David Ralph
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  2. Michael McClelland
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Editors and Affiliations

  1. Central Public Health Laboratory, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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© 1998 Humana Press Inc.

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Ralph, D., McClelland, M. (1998). Arbitrarily Primed PCR Methods for Studying Bacterial Diseases. In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:83

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  • DOI: https://doi.org/10.1385/0-89603-498-4:83

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-498-3

  • Online ISBN: 978-1-59259-599-0

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