Abstract
RNA dot hybridizations were first described by Kafatos et al. (1). They enable rapid detection of transcription from a number of different mRNA populations and are particularly useful in the initial characterization of cDNA clones isolated by differential screening. In cases in which many samples have to be handled, filtration manifold systems are available, such as the Millipore MilliBlot system, which use a vacuum source to transfer nucleic acid to filter.
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References
Kafatos F. C, Jones C W., and Efstratiadis A. (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acid Res. 7, 154l–l552.
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© 1998 Humana Press Inc.
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Hodge, R. (1998). Preparation of RNA Dot-Blots. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:73
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DOI: https://doi.org/10.1385/0-89603-494-1:73
Publisher Name: Humana Press
Print ISBN: 978-0-89603-494-5
Online ISBN: 978-1-59259-570-9
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