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Formaldehyde Gel Electrophoresis of Total RNA

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 86)

Abstract

RNA has the tendency to form both secondary and tertiary structures that can Impede its separation by electrophoresis. As such, identical species of RNA exhibiting varying degrees of intramolecular base-pairing, migrate at different rates and result in the smearing of distinct RNA molecules. Consequently, the electrophoresis of RNA needs to be performed under denaturing conditions. Heat denaturing the RNA sample prior to electrophoresis is insufficient, as secondary structures will simply reform unless a denaturing system is used.

Keywords

  • Bromophenol Blue
  • Fume Hood
  • Propanesulfonic Acid
  • Ethidium Bromide Solution
  • Localize Smear

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Boedtker, H. (1971) Conformation-independent molecular determmations of RNA by gel electrophoresis. Biochim. Biophys Acta. 240, 448

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  3. Manning, D. L., McClelland, R. A., Gee, J. M, Chan, C. M., Green, C. D., Blarney, R. W, and Nicholson, R. I (1993) The role of four estrogen responsive genes pLIV1, pS2, PSYD3, and pSYD8 in predictmg responsiveness to therapy in primary breast cancer. Eur J Cancer 29(10), 1462–1468.

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© 1998 Humana Press Inc.

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Bryant, S., Manning, D.L. (1998). Formaldehyde Gel Electrophoresis of Total RNA. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:69

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  • DOI: https://doi.org/10.1385/0-89603-494-1:69

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-494-5

  • Online ISBN: 978-1-59259-570-9

  • eBook Packages: Springer Protocols