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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 86))

Abstract

mRNA comprises approx l–5% of total cellular RNA. Although the actual amount depends on the type of cell and its physiological state, at any given time approx 12,000 genes are being transcribed with approx 500,000 mRNA molecules present in each mammalian cell.

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References

  1. Manning D. L., Daly R. J, Lord P. G., Kelly K. F., and Green C. D. (1988) Effects of Estrogen on the expression of a 4.4 kb mRNA in the ZR-75-1 human breast cancer cell line Mol. Cell Endo 59 205–212.

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  2. Manning D. L., Archibald L. H., and Ow K. T. (1990) Cloning of estrogenresponsive mRNAs in the T-47D human breast cancer cell line Cancer Res 50 4098–4104

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© 1998 Humana Press Inc.

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Bryant, S., Manning, D.L. (1998). Isolation of Messenger RNA. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:61

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  • DOI: https://doi.org/10.1385/0-89603-494-1:61

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-494-5

  • Online ISBN: 978-1-59259-570-9

  • eBook Packages: Springer Protocols

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