In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
The in vtvo competition of mRNAs for translation IS more accurately represented, making the wheat germ system preferable for studymg regulation of translation (1).
Particularly low levels of endogenous mRNA and the endogenous nuclease activity (10) obviate the requirement for treatment with a calcmm-activated nuclease. There IS, therefore, less dlsruption of the in vivo situation and contamination with calcium ions is less harmful The ldentlfication of all sizes of exogenous mRNA-directed translation products IS faclhtated because of the low levels of endogenous mRNA present.
There is no posttranslational modification of translation products; primary products are therefore investigated, although processmg may be achieved by the addition of mlcrosomal membranes to the translation reaction.
KeywordsCreatine Kinase Wheat Germ Translation Product Translation System Microsomal Membrane
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