Phospholipid Signaling Protocols pp 77-87 | Cite as
Inositol 1,4,5-Trisphosphate Mass Assay
Protocol
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Abstract
The phosphoinositide cascade plays a central role in the transduction of many extracellular stimuli. Activation of plasma membrane-associated phosphoinositide-specific phospholipase C stimulates the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdInsP2) and generates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), which in turn releases calcium from intracellular stores (1).
Keywords
Inositol Phosphate Scintillation Cocktail Bovine Serum Albumin Solution Assay Protocol Radioreceptor Assay
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© Humana Press Inc., Totowa, NJ 1998