Abstract
Differential display is a powerful method to identify genes that are differentially expressed in different tissues or in the same tissue exposed to different treatments (1,2). The method can be performed with relatively little biological material, and the resulting partial cDNAs can be cloned, sequenced, and used for subsequent expression studies. cDNA fragments derived from downregulated as well as upregulated transcripts can be detected simultaneously, and cDNAs corresponding to messages of high and low abundance are detected with similar probabilities.
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References
Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971.
Liang, P., Averboukh, L., and Pardee A. B. (1993) Distribution and cloning of mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res. 21, 3269–3275.
Chen, Z., Swisshelm, K., and Sager, R. (1994) A cautionary note on the reaction tubes for differential display and cDNA amplification in thermal cycling. BioTechniques 16, 1003–1006.
Li, F., Barnathan, E. S., and Kariko, K. (1994) Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of Northern blot for affinity capturing of cDNAs. Nucleic Acids Res. 22, 1764,1765.
Callard, D., Lescure, B., and Mazzolini, L. (1994) A method for the elimination of false positives generated by the mRNA differential display technique. BioTechniques 16, 1096–1103.
Vögeli-Lange, R., Bürckert, N., Boiler, T., and Wiemken, A. (1996) Rapid selection and classification of positive clones generated by mRNA differential display. Nucleic Acids Res. 24, 1385, 1386.
Liang, P., Zhu, W., Zhang, X., Guo, Z., O’Connell, R. P., Averboukh, L., Wang, F., and Pardee, A. B (1994) Differential display using one-base anchored oligo-dT primers. Nucleic Acids Res. 22, 5763, 5764.
Yeatman, T. J. and Mao, W. (1995) Identification of a differentially-expressed message associated with colon cancer liver metastasis using an improved method of differential display. Nucleic Acids Res. 23, 4007, 4008.
Trentmann, S. M., van der Knaap, E., and Kende, H. (1995) Alternatives to 35S as a label for the differential display of eukaryotic messenger RNA. Science 267, 1186.
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© 1997 Humana Press Inc.
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Vögeli-Lange, R., Bürckert, N., Boller, T., Wiemken, A. (1997). Screening for Positive Clones Generated by Differential Display. In: Liang, P., Pardee, A.B. (eds) Differential Display Methods and Protocols. Methods in Molecular Biology, vol 85. Humana Press. https://doi.org/10.1385/0-89603-489-5:95
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DOI: https://doi.org/10.1385/0-89603-489-5:95
Publisher Name: Humana Press
Print ISBN: 978-0-89603-489-1
Online ISBN: 978-1-59259-569-3
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