Abstract
One of the greatest unsolved mysteries of life is how the hundreds of thousands of genes embedded in the genome of an organism are selectively expressed into the mRNA and proteins in a temporally and spatially regulated manner that gives rise to different tissues and organs. The abnormality in this intricate regulatory circuitry is believed to be one of the underlining causes of a variety of pathological alterations or disease states. The isolation and characterization of differentially expressed genes becomes one of the first steps toward the understanding of these important biological questions. Differential display (1) and a related RAP-PCR method (2) were developed to more efficiently identify and isolate these genes.
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References
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© 1997 Humana Press Inc.
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Liang, P., Pardee, A.B. (1997). Differential Display. In: Liang, P., Pardee, A.B. (eds) Differential Display Methods and Protocols. Methods in Molecular Biology, vol 85. Humana Press. https://doi.org/10.1385/0-89603-489-5:3
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DOI: https://doi.org/10.1385/0-89603-489-5:3
Publisher Name: Humana Press
Print ISBN: 978-0-89603-489-1
Online ISBN: 978-1-59259-569-3
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