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Overlay and Bead Assay

Determination of Calcium Channel Subunit Interaction Domains

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Protein Targeting Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 88))

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Abstract

Based on electrophysiological and pharmacological properties, voltage-dependent Ca2+ channels are classified as L-, N-, T-, and P/Q- type Ca2+ channels (1,2). The brain N-type Ca2+ channels (3) and the skeletal muscle L-type (4,5) have both been purified and shown to have a similar subunit composition (α1-, α2/δ-, and β-subunits).

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© 1998 Humana Press Inc.

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Scott, V.E.S., Gurnett, C.A., Campbell, K.P. (1998). Overlay and Bead Assay. In: Clegg, R.A. (eds) Protein Targeting Protocols. Methods in Molecular Biology™, vol 88. Humana Press. https://doi.org/10.1385/0-89603-487-9:71

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  • DOI: https://doi.org/10.1385/0-89603-487-9:71

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-487-7

  • Online ISBN: 978-1-59259-572-3

  • eBook Packages: Springer Protocols

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