Abstract
Size-exclusion chromatography (gel filtration) is a widely used method for the estimation of protein mol wt (M r). Although, strictly speaking, the technique measures not M r as such, but a property known as hydrodynamic radius (1), the correlation between the two is such that this distinction has all but disappeared from the literature. Gel filtration has the advantage over other methods for determining protein M r, in that it is typically a nondenaturing methodology and therefore reports on native protein M rs. In addition, the nondenaturing nature of the technique allows for the study of protein-protein interactions (e.g., enzyme-substrate, hormone-receptor, and protein subunits) that occur normally in vivo. Therefore, provided that an appropriate reporting assay is available, this technique can be employed to examine a wide range of protein interactions of biological significance. The widespread use and availability of high-performance liquid chromatography (HPLC) and fast-protein liquid chromatography (FPLC) systems allows for the rapid and reproducible generation and analysis of data.
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© 1998 Humana Press Inc.
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Beattie, J. (1998). Size-Exclusion Chromatography. In: Clegg, R.A. (eds) Protein Targeting Protocols. Methods in Molecular Biology™, vol 88. Humana Press. https://doi.org/10.1385/0-89603-487-9:65
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DOI: https://doi.org/10.1385/0-89603-487-9:65
Publisher Name: Humana Press
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