Abstract
The ability to express recombinant proteins in eukaryotic systems by transiently expressing cloned cDNAs in cell lines, such as COS-1, COS-7 (1), or human embryonic kidney HEK293 cells (2), has enabled rapid analysis of a variety of wild-type and mutant signal transduction polypeptides. These have included receptors, G proteins (α-, β-, andγ-subunits), and effector molecules. This chapter aims to outline the techniques used in such experiments and to highlight potential problems, which, unless specifically investigated, may not normally be appreciated.
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© 1998 Humana Press Inc.
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Grassie, M.A., Milligan, G. (1998). Molecular Genetic Approaches IV. In: Clegg, R.A. (eds) Protein Targeting Protocols. Methods in Molecular Biology™, vol 88. Humana Press. https://doi.org/10.1385/0-89603-487-9:151
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DOI: https://doi.org/10.1385/0-89603-487-9:151
Publisher Name: Humana Press
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