Skip to main content
SpringerLink
Log in

Methods for Producing High Titer, Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cells

  • Protocol
Book cover Gene Therapy Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 7))

  • 632 Accesses

Abstract

Current limitations to the use of Moloney murine leukemia virus (MoMLV)-derived retroviral vectors as a tool for gene transfer include the inability to obtain high-titer vector stocks and the narrow host cell range of these vectors. To overcome these disadvantages, we developed a new class of pantropic retroviral vector that has a broadened host cell range and can be concentrated to very high titers (>109 colony forming units [CFU]/mL) (1)

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Burns, J. C, Friedmann, T., Dreiver, W., Burrascano, M., and Yee, J.-K. (1993) Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90, 8033–8037.

    Article  CAS  Google Scholar 

  2. van Zeijl, M., Johann, S. V., Closs, E., Cummingham, J., Eddy, R., Shows, T. B., and O’Hara, B. (1994) A human amphotropic retrovirus receptor is a second member of the gibbon ape leukemia virus receptor family. Proc. Natl Acad. Sci USA 91, 1168–1172.

    Article  Google Scholar 

  3. Mastromarino, P., Conti, C., Goldoni, P., Hauttecoeur, B., and Orsi, N. (1987) Characterization of membrane components of the erythrocyte involved in vesicular stomatitis virus attachment and fusion at acidic pH. J. Gen Erol. 68, 2359–2369.

    CAS  Google Scholar 

  4. Burns, J C., Matsubara, T., Lozinski, G., Yee, J.-K., Friedmann, T., Washabaugh, C. H., and Tsonis, P. A. (1994) Pantropic retroviral vector-mediated gene transfer, integration, and expression in cultured newt limb cells. Develop Biol. 165, 285–289.

    Article  CAS  Google Scholar 

  5. Lin, S., Gaiano, N., Culp, P., Burns, J. C., Friedmann, T., Yee, J.-K., and Hopkins, N. (1994) Integration and germ-line transmission of a pseudotyped retroviral vector in zebrafish. Science 265, 666–668.

    Article  CAS  Google Scholar 

  6. Miller, A. D. and Buttimore, C. (1986) Redesign of retrovirus packaging lines to avoid recombination leading to helper virus production. Mol. Cell. Biol 6, 2895–2902.

    CAS  Google Scholar 

  7. Quade, K. (1979) Transformation of mammalian cells by avian myelocytomatosis virus and avian erythroblastosis virus. Virology 98, 416–465.

    Article  Google Scholar 

  8. Miyanohara, A., Elam, R. L., Witztum, J. L., and Friedmann, T. (1992) Long-term transgene expression for genetically modified hepatocytes grafted to the rat liver. New Biologist 4, 261–267.

    CAS  Google Scholar 

  9. Graham, F. L. and van der Eb, A. J. (1973). A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, School of Medicine, University of California, San Diego, CA, USA

    Jane C. Burns

  2. Department of Pediatrics, City of Hope, Duarte, CA, USA

    Jiing-Kuan Yee

  3. Department of Pediatrics, University of California at San Diego School of Medicine, La Jolla, CA, USA

    Alice L. Yu

Authors
  1. Jane C. Burns
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiing-Kuan Yee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alice L. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Pittsburgh, PA, USA

    Paul D. Robbins

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Burns, J.C., Yee, JK., Yu, A.L. (1997). Methods for Producing High Titer, Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cells. In: Robbins, P.D. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 7. Humana, Totowa, NJ. https://doi.org/10.1385/0-89603-484-4:213

Download citation

  • DOI: https://doi.org/10.1385/0-89603-484-4:213

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-484-6

  • Online ISBN: 978-1-59259-591-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation