Abstract
The rapid amplification and isolation of specific DNA fragments made possible by the polymerase chain reaction (1,2) has become routine for a variety of molecular biology studies and applications. Taq DNA polymerase is still the most widely used enzyme for PCR amplifications, despite the fact that a number of other thermostable DNA polymerases are now available from commercial suppliers. Taq DNA polymerase is capable of catalyzing the addition of a single nucleotide, a deoxyadenosine (dA), to the 3′ ends of amplified PCR products (3), resulting in a single nucleotide 3′ overhang. This renders blunt-end ligation of such PCR products to cloning vectors inefficient.
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© 1997 Humana Press Inc.
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Testori, A., Sollitti, P. (1997). Cloning Unmodified PCR Products Using Engineered Xcml Restriction Sites in a Portable Cassette. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:89
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DOI: https://doi.org/10.1385/0-89603-483-6:89
Publisher Name: Humana Press, Totowa, NJ
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