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Rapid Amplification of cDNA Ends

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 67)


Rapid amplification of cDNA ends (RACE) allows the amplification of either the 5′ or 3′ end of a specific cDNA starting from a mRNA population (5′-RACE and 3′-RACE respectively: refs. 1,2). The method allows the amplification of regions with unknown sequence and only requires the knowledge of a single short sequence within the mRNA of interest. The method is often used for cloning the remainder of a cDNA using a sequence from an incomplete cDNA. Incomplete cDNAs are frequently obtained by, for instance, the screening of a library or from PCR using degenerate primers.


  • Structural RNAs
  • General Primer
  • Restriction Endonuclease Site
  • Hexadecyltrimethylammonium Bromide
  • Terminal Transferase

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© 1997 Humana Press Inc.

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Bertioli, D. (1997). Rapid Amplification of cDNA Ends. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ.

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-483-9

  • Online ISBN: 978-1-59259-553-2

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