Rapid amplification of cDNA ends (RACE) allows the amplification of either the 5′ or 3′ end of a specific cDNA starting from a mRNA population (5′-RACE and 3′-RACE respectively: refs. 1,2). The method allows the amplification of regions with unknown sequence and only requires the knowledge of a single short sequence within the mRNA of interest. The method is often used for cloning the remainder of a cDNA using a sequence from an incomplete cDNA. Incomplete cDNAs are frequently obtained by, for instance, the screening of a library or from PCR using degenerate primers.
- Structural RNAs
- General Primer
- Restriction Endonuclease Site
- Hexadecyltrimethylammonium Bromide
- Terminal Transferase
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© 1997 Humana Press Inc.
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Cite this protocol
Bertioli, D. (1997). Rapid Amplification of cDNA Ends. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:233
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-483-9
Online ISBN: 978-1-59259-553-2
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