Abstract
Most commonly used prokaryotic expression vectors fall into one of the following three categories:
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1.
They have a low copy number and a low yield of single-stranded DNA,
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2.
They have a high copy number and the ability to produce a high yield of single-stranded DNA, but require a suitable host to achieve an inducible protein expression; or
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3.
They lack the ability to produce single-stranded DNA.
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© 1997 Humana Press Inc.
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Garcia, G.A., Chong, S. (1997). Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7. In: Tuan, R.S. (eds) Recombinant Gene Expression Protocols. Methods in Molecular Biology, vol 62. Humana Press. https://doi.org/10.1385/0-89603-480-1:63
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DOI: https://doi.org/10.1385/0-89603-480-1:63
Publisher Name: Humana Press
Print ISBN: 978-0-89603-480-8
Online ISBN: 978-1-59259-548-8
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