Abstract
As a result of morphological and developmental characteristics of the early avian embryo, several approaches, taking place at early stages, have been investigated to introduce foreign DNA into the avian genome. They include:
-
1.
Insemination with sperm cells to which foreign DNA was coated (1,2);
-
2.
Transfection of DNA into follicles before fertilization (3),
-
3.
Direct injection of naked DNA into newly fertilized ova or into germinal disk (4–7),
-
4.
Microinjection of replication-defective retroviral vectors (8-10) into unincubated embryo blastoderm, and;
-
5.
Retroviral infection of primordial germ cells, PGCs (11,12).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gavora, J. S., Benkel, B., Sasada, H., Cantwell, W. J., Fiser, P., Teather, R. M., Nagai, J., and Sabour, M. P. (1991) An attempt at sperm-mediated gene transfer in mice and chickens. Can. J. Anim. Sci. 71, 287–291.
Rottman, O. J., Antes, R., Hofer, P., and Maierhofer, G. (1992) Liposome mediated gene transfer via spermatozoa into avian egg cells. J. Anim. Breed Genet. 109, 64–70.
Perry, M., Morrice, D., Hettle, S., and Sang, H. (1991) Expression of exogenous DNA during the early development of the chick embryo. Roux’s Arch. 179, 85–110.
Naito, M., Agata, K., Otsuka, K., Kino, K., Ohta, M., Hirose, K., Perry, M. M., and Eguchi, G. (1991) Embryonic expression of the β-actin-lacZ hybrid gene injected into the fertilized ovum of the domestic fowl. Int. J. Dev. Biol. 35, 69–75.
Sang, H. and Perry, M. M. (1989) Episomal replication of cloned DNA injected into the fertilized ovum of the hen, gallus domesticus. Mol. Rep. Dev. 1, 98–106.
Shuman, R. M. (1986) Gene transfer in avian species using retrovirus vectors 7th European Poultry Conference, (Parts. Ed World’s Poultry Science Association) 1, 24–37.
Love, J., Gribbin, C., Mather, C., and Sang, H. (1994) Transgenic birds by DNA microinjection. Biotechnology 12, 60–63.
Bosselman, R. A., Hsu, R. Y., Briskin, M. J., Boggs, T., Hu, S., Nicolson, M., Souza, L. M., Schultz, J. A., Rishell, W., and Stewart, R. G. (1990) Transmission of exogenous genes into the chicken. J. Reprod. Fert. 41, 183–195.
Bosselman, A. R., Hu, R. Y., Boggs, T., Hu, S., Bruszewski J., Ou, S., Kozar, L., Martin, F., Green, C., Jacobsen, F., Nicolson, M., Schultz, J. A., Semon, K. M., Rishell, W., and Stewart, R. G. (1989) Germline transmrsslon of exogenous genes in chicken. Science 243, 533–535.
Thoraval, P., Afanassieff, M., Cosset, F. L., Lasserre, F., Verdier, G., and Dambrine, G. (1995) Germ line transmission of exogenous genes in chicken by use of helper free ecotropic avian leukosis viruses based vectors. Transgenic Research 4, 369–376.
Simkiss, K., Rowlett, K., Bumstead, N., and Freeman, B. M. (1989) Transfer of primordial germ cell DNA between embryos. Protoplasma 151, 164–166.
Vick, L., Li, Y., and Simkiss, K. (1993) Transgenic birds from transformed primordial germ cells. Proc. R. Soc. Lond. B. 251, 179–182.
Wentworth, B. C., Tsai, F., Hallett, J. H., Gonzales, D. S., and Rajcic-Spasojevic, G. (1989) Manipulation of avian primordial germ cells and gonadal differentiation. Poultry Sc. 68, 999–1010.
Kuwana, T. and Fujimoto, T. (1984) Locomotion and scanning election microscopic observations of primordial germ cells from the embryonic chick blood in vitro. Anat. Rec. 209, 337–343.
Allioli, N., Thomas, J. L., Chebloune, Y., Nigon, V. M., Verdier, G., and Legras, C. (1994) Use of retroviral vectors to introduce and express the beta galactosidase marker gene in cultured chicken primordial germ cells. Devel. Biol. 165, 30–37.
Guido, T. C., Abott, U. K., and Carrey, J. R. (1991) The interspecific transfer of avian primordial germ cells. Manipulation of the avian genome. Keystone symposia on Molecular and Cellular Biology. J. Cell Biochem. supp15E, 204.
Cosset, F. L., Legras, C., Thomas, J. L., Molina, R. M., Chebloune, Y., Faure, C., Nigon, V. M., and Verdier, G. (1991) Improvement of avian leukosis virus (ALV)-based retroviruses vectors by using different cis-acting sequences from ALVs. J. Virol. 65, 3388–3394.
Cosset, F. L., Ronfort, C., Molina, R. M., Flamant, F., Drynda, A., Benchaibi, M., Valsesia, S., Nigon, V. M., and Verdier, G. (1992) Packaging cells for avian leukosis virus-based vectors with various host ranges. J. Virol. 66, 5671–5676.
Hamburger, V. and Hamilton, H. L. (1951) A series of normal stages in the development of the chick embryo. J. Morphol. 88, 49–92.
Cosset, F. L., Legras, C., Chebloune, Y., Savatier, P., Thoraval, P., Thomas, J. L., Samarut, J., Nigon, V. M., and Verdier, G. (1990) A new avian leukosis virus (ALV)-based packaging cell line using two separate complementing helper genomes. J. Virol. 64, 1070–1078.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Humana Press Inc.
About this protocol
Cite this protocol
Allioli, N., Verdier, G., Legras, C. (1997). Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens. In: Tuan, R.S. (eds) Recombinant Gene Expression Protocols. Methods in Molecular Biology, vol 62. Humana Press. https://doi.org/10.1385/0-89603-480-1:425
Download citation
DOI: https://doi.org/10.1385/0-89603-480-1:425
Publisher Name: Humana Press
Print ISBN: 978-0-89603-480-8
Online ISBN: 978-1-59259-548-8
eBook Packages: Springer Protocols