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Design and Construction of Recombinant Vaccinia Viruses

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Recombinant Gene Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 62))

Abstract

The structural and functional analyses of proteins have benefited enormously from the use of technologies of recombinant gene expression. The recombinant vaccinia virus system has been widely employed to express genes from eukaryotic, prokaryotic, and viral origins (for reviews, see refs. 2–7) and several detailed protocols for the generation, identification, isolation, and characterization of recombinant vaccinia viruses have been published (8-10).

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Authors and Affiliations

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD

    Christopher C. Broder & Patricia L. Earl

Authors
  1. Christopher C. Broder
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  2. Patricia L. Earl
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Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

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© 1997 Humana Press Inc.

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Broder, C.C., Earl, P.L. (1997). Design and Construction of Recombinant Vaccinia Viruses. In: Tuan, R.S. (eds) Recombinant Gene Expression Protocols. Methods in Molecular Biology, vol 62. Humana Press. https://doi.org/10.1385/0-89603-480-1:173

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  • DOI: https://doi.org/10.1385/0-89603-480-1:173

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-480-8

  • Online ISBN: 978-1-59259-548-8

  • eBook Packages: Springer Protocols

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