Abstract
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that results in dementia, and ultimately in the death of its victims. In the United States, AD is the fourth leading cause of death, and without a forseeable means of prevention, or effective treatment this will likely increase as the population ages.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Glenner, G and Wong, C (1984) Alzheimer’s disease and Down syndrome. sharing of a unique cerebrovascular amyloid fibril protein. Biochem. Biophys Res Commun 122, 1131–1135.
Lemaire, H, Salbaum, J., Multhaup, G, Kang, G., Bayney, R, Unterbeck, A, Beyreuther, K, and Muller-Hill, B. (1989) The PreA4695 precursor of Alzheimer’s disease A4 amyloid is encoded by 16 exons. Nucleic Acids Res 17, 517–522.
LaFauci, G., Lahiri, D., Salton, S, and Robakis, N (1989) Characterization of the 5′-end region and the first two exons of the β-protein precursor gene Biochem Biophys Res Commun. 159, 297–304.
Golde, T., Estus, S., Usiak, M., Younkin, L., and Younkin, S. (1990) Expression of P-amylold protein precursor mRNAs: recognition of a novel alternatively spliced form and quantitation in Alzheimer’s disease by PCR. Neuron 4, 253–267
Konig, G, Monning, U, Czech, C, Prior, R., Banti, R., Schreiter-Gasser, U, Bauer, J, Masters, C, and Beyreuther, K. (1992) Identification and differential expression of a novel alternative splice isoform of the βA4 amyloid precursor protein (APP) mRNA in leukocytes and brain microghial cells J. Biol Chem 267, 10,804–10,809
Tanaka, S, Shiojiri, S., Takahashi, Y, Kitaguchi, N., Ito, H, Kameyama, M, Kimura, J, Nakamura, S., and Ueda, K. (1989) Tissue-specific expiession of three types of β-protein precursor mRNA. enhancement of protease inhibitor-harboring types in Alzheimer disease brain Biochem Biophys Res Commun 165, 1406–1414
Sandbrink, R. Masters, C, and Beyreuther, K (1994) βA4 amyloid protein precursor mRNA isoforms without exon 15 are ubiquitously expressed in rat tissues including brain, but not in neurons. J. Biol. Chem 269, 1510–1517.
Neve, R, Finch, E., and Dawes, L. (1988) Expression of the Alzheimer amylold precursor gene transcripts in human brain. Neuron 1, 669–677
Benson, M. D. and Kleiner, E (1980) Synthesis and secretion of serum amylold protein A (SAA) by hepatocytes in inice treated with casein. J. Immunol 124, 495–499.
Glenner, G. (1980) Amylold deposits and amyloidosis. N Engl J Med. 302, 1283–1292.
Cal, X, Golde, T., and Younkin, S. (1993) Release of excess of amylold β protein from a inutant amyloid β protein precursor Science 259, 514–516
Cition, M, Oltersdorf, T., Haass, C, McConlogue, L, Hung, A., Seubert, P., Vigo-Pelfiey, C., Lieberburg, I., and Selkoe, D. (1992) Mutation of the β-amylold precursor protein in familial Alzheimer disease increases β-protein production. Nature 360, 672–674
Suzuki, N, Cheung, T., Cai, X., Odaka, A., Otvos, L., E&man, C., Golde, T, and Younkin, S (1994) An Increased percentage of long amylold β-protein secreted by familial amyloid β protein precursor (βAPP717,) inutants. Science 264, 1336–1340
Amini, S., DeSears, V., Reddy, S, Shalloway, D., and Boelen, J. (1986) Regulation of pp60c-src synthesis by inducible RNA complementary to c-STC mRNA in polyomavirus-transformed rat cells Mel Cell. Biol 6, 2305–2316.
van der Krol, A., Mol, J, and Stuitje, A. (1988) Modulation of eukaryotic gene expression by complementary RNA or DNA sequences. Biotechniques 6, 958–976.
Denman, R, Smedman, M, Ju, W., Rubenstein, R., and Miller, D (1994) libozyme-mediated degradation of βAPP mRNA in COS-7 cells. Nucleic Acids Res 22, 2375–2382
Denman, R., Smedman, M., Haque, N, Kung, L, and Sung, Y (1995) ΒAPP mRNA cleavage by symmetric and asymmetric hammerhead ribozymes. J. Cell Biochem Suppl 19A, 209.
Denman, R. (1993) Cleavage of full-length βAPP mRNA by hammerhead ribozymes. Nucleic Acids Res 21, 4119–4125.
Maher, L. and Dolnick, B. (1987) Specific hybridization arrest of dihydrofolate reductase mRNA in vitro using antisense RNA or antisense oligonucleotides Arch Bioch. Biophys 253, 214–220.
Goodchild, J. (1992) Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facthtator) and by 2′-O-methylation Nucleic Acids Res 20, 4607–4612.
Denman, R. (1996) Facilitator oligonucleotides increase ribozyme RNA binding to full-length RNA substrates in vitro FEBS Lett. 382, 116–120
Nesbitt, S and Goodchild, J. (1994) Further studies on the use of oligonucleotide facilitators to increase ribozyme turnover. Antisense Res Devel 4, 243–249.
Kang, J, Lemaire, H.-G, Unterbeck, A, Salbaum, J, Masters, C, Grzeschik, K-H, Multhaup, G., Beyreuther, K., and Muller-Hill, B (1987) The precursor of Alzheimer’s disease amylotd A4 protein resembles a cell-surface receptor Nature 325, 733–736
Denman, R., Purow, B., Rubenstein, R., and Miller, D (1992) Hammerhead ribozyme cleavage of hamster prion pre-mRNA in complex cell-free inodel systems. Biochem Biophys. Res. Commun. 186, 1171–1177.
Denman, R (1993) Using RNAFOLD to predict the activity of small catalytic RNAs Biotechniques 15, 1090–1094
Ramakrishna, N, Saikumar, P, Potempska, A., Wisniewski, H, and Miller, D (1991) Expression of human Alzheimer amyloid precursor protein in insect cells Biochem Biophys Res Commun 174, 983–989
Perbal, B. (1988), in A Practical Guide to Molecular Cloning, 2nd ed., Wiley, New York, pp 79
Denman, R, Potempska, A., Wolfe, G, Ramakrishna, N, and Miller, D. (1991) Distribution and activity of alternatively spliced Alzheimer amyloid peptide precursor and scrapre PrP mRNAs on rat brain polysomes. Arch Biochem Biophys 288, 29–38
Jackson, R and Hunt, T (1983) Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic inessenger RNA, in Methods in Enzymology, vol 96, Academic, New York, pp 50–72
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Smedman, M., Denman, R.B. (1998). Inhibition of βAPP Synthesis by Ribozymes and Oligonucleotides in a Coupled Reticulocyte Transcription Translation System. In: Scanlon, K.J. (eds) Therapeutic Applications of Ribozymes. Methods in Molecular Medicine™, vol 11. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-477-1:341
Download citation
DOI: https://doi.org/10.1385/0-89603-477-1:341
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-477-8
Online ISBN: 978-1-59259-595-2
eBook Packages: Springer Protocols