Abstract
The chemical composition of human blood plasma is widely studied and well-known, however, the exact nature of the antioxidants of plasma is very much open to dispute. The study of plasma antioxidants has been given a fresh stimulus in recent years by the development of new methods for the measurement of hydrogen-donating antioxidant activity, the majority using fluorescent or chemiluminescent endpoints. The feature that these systems have in common with the older techniques (1) is that they are all inhibition assays (Fig. 1). In these assays, a free radical is generated and its generation is then linked to an endpoint that can be observed and quantified. Addition of an antioxidant inhibits the development of this endpoint and in each case from this measured inhibition, the antioxidant activity is quantitated (2).
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References
Rice-Evans, C and Miller, N. J (1994) Total antioxidant status in plasma and body fluids Methods Enzymol 234, 279–293
Miller, N. J. and Rice-Evans, C. A (1996) Spectrophotometric determination of antioxidant activity. Redox Rep. 2, 161–171
Wayner, D. D. M., Burton, G W., Ingold, K U, and Locke, S. (1985) Quantitative measurement of the total peroxyl radical-trapping antioxidant capability of human blood plasma by controlled peroxidation: the important contribution made by human plasma proteins. FEBS Lett. 187, 33–37.
Metsa-Ketela, T (1991) Luminescent assay for total peroxyl radical-trapping antioxidant activity of human blood plasma, in Bioluminescence and Chemiluminescence. Current Status (Stanley, P and Kricka, L, eds), John Wiley and Sons, Chichester, UK, pp 389–392.
Metsa-Ketela, T and Kirkkola, A.-L (1992) Total peroxyl radical-trapping capability of human LDL Free Rad. Res. Commun. 16S, 215
Lissi, E., Salim-Hanna, M, Pascual, C, and Del Castillo, M D (1995) Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurements. Free Rad Biol. Med. 18, 153–158
Delange, R J. and Glazer, A N. (1989) Phycoerythnn fluorescence-based assay for peroxyl radicals: a screen for biologically relevant protective agents Anal Biochem. 177, 300–306.
Cao, G., Alessio, H M, and Cutler, R G (1993) Oxygen-radical absorbance capacity assay for antioxidants. Free Rad Biol. Med 14, 303–311.
Ghiselh, A, Serafini, M, Maiam, G, Azzim, E., and Ferro-Luzzi, A (1995) A fluorescence-based method for measuring total plasma antioxidant capability Free Rad Biol Med. 18, 29–36
Laranjinha, J A N, Almeida, L M., and Madeira, V M C (1994) Reactivity of dietary phenolic acids with peroxyl radicals: antioxidant activity upon low density lipoprotein oxidation. Biochem Pharmacol 48, 487–494
McKenna, R., Kezdy, F. J, and Epps, D E. (1991) Kinetic analysis of the free-radical-induced lipid peroxidation in human erythrocyte membranes, evaluation of potential antioxidants using cis-pannanc acid to monitor peroxidation. Anal. Biochem 196, 443–450.
Pryor, W A, Cornicelh, J A, Devall, L J, Tait, B, Tnvedi, B K, Witiak, D T, and Wu, M (1993) A rapid screening test to determine the antioxidant potencies of natural and synthetic antioxidants J Org. Chem. 58, 3521–3532
Flecha, B G, Llesuy, S., and Boveris, A (1991) Hydroperoxide-imtiated chemiluminescence: an assay for oxidative stress in biopsies of heart, liver and muscle Free Rad Biol Med 10, 93–100
Whitehead, T P, Thorpe, G H.G, and Maxwell, S.R J. (1992) Enhanced chemiluminescent assay for antioxidant capacity in biological fluids. Anal. Chim. Acta 266, 265–277
Lewin, G. and Popov, I. (1994) Photochemiluminescent detection of antiradical activity, III: a simple assay of ascorbate in blood plasma J Biochem Biophys Methods 28, 277–282
Negre-Salvayre, A., Alomar, Y., Troly, M., and Salvayre, R. (1991) Ultraviolet-treated lipoproteins as a model system for the study of the biological effects of lipid peroxides on cultured cells The protective effect of antioxidants (probucol, catechin, vitamin E) against the cytotoxicity of oxidized LDL occurs in two different ways. Biochim. Biophys Acta. 1096, 291–300.
Yuting, C, Rongliang, Z., Zhongjian, J., and Yong, J (1990) Flavonoids as superoxide scavengers and antioxidants. Free Rod. Biol. Med. 9, 19–21
Jan, C. Y, Takahama, U, and Kimura, M. (1991) Inhibition of photooxidation of a-tocopherol by quercetin in human blood cell membranes in the presence of haematoporphyrin as a sensitiser. Biochim. Biophys. Acta. 1086, 7–14.
Miller, N. J., Rice-Evans, C, Davies, M. J, Gopinathan, V., and Milner, A. (1993) A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci. 84, 407–412.
He, X M and Carter, D. C (1992) Atomic structure and chemistry of human serum albumin. Nature 358, 209–215
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© 1998 Humana Press Inc., Totowa, NJ
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Miller, N.J. (1998). Nonvitamin Plasma Antioxidants. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:285
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DOI: https://doi.org/10.1385/0-89603-472-0:285
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