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Oxidized and Unoxidized Fatty Acyl Esters

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Free Radical and Antioxidant Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 108))

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Abstract

Membrane phospholipids contain polyunsaturated fatty-acid side chains esterified to phosphoglycerol. These polyunsaturated fatty acyl esters are easily oxidable lipids. A sensitive method for determination of lipid peroxidation is of considerable interest. Usually lipid peroxidation is determined by evaluation of a by-product, malondialdehyde, which is produced by lipid-peroxide breakdown. Malondialdehyde, however, does not represent the early stage of membrane lipid peroxidation nor the true amount of oxidized lipids. Moreover, most techniques for malondialdehyde use thiobarbituric acid-reactants evaluation (1), which is an indirect measurement.

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© 1998 Humana Press Inc., Totowa, NJ

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Sergent, O., Cillard, J. (1998). Oxidized and Unoxidized Fatty Acyl Esters. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:131

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  • DOI: https://doi.org/10.1385/0-89603-472-0:131

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-472-3

  • Online ISBN: 978-1-59259-254-8

  • eBook Packages: Springer Protocols

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