Abstract
Protein phosphatase 2A (PP2A) is one of the major intracellular serine/threonine protein phosphatases. PP2A in vivo is an ABC heterotrimer whose substrate specificity is regulated in large part by the variable B subunit. Several additional polypeptides have been identified that modulate the activity of the heterotrimer. It seems likely that more regulatory subunits and interacting polypeptides exist that may be of low abundance or expressed in a tissue-specific manner. One attractive approach to finding these novel interacting polypeptides is to utilize the two-hybrid interaction method.
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McCright, B., Virshup, D.M. (1998). Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-Hybrid Method. In: Ludlow, J.W. (eds) Protein Phosphatase Protocols. Methods in Molecular Biology™, vol 93. Humana Press. https://doi.org/10.1385/0-89603-468-2:263
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DOI: https://doi.org/10.1385/0-89603-468-2:263
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