DNase I Footprinting

  • Keith R. Fox
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 90)

Abstract

Footprinting provides a simple, quick, and reasonably inexpensive method for assessing the sequence specific interaction of ligands with DNA. Although the technique was developed in 1978 for studying the interaction of DNA-binding proteins with then target sites (1), it has proved invaluable for determining the sequence specificity of many small ligands

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Copyright information

© Humana Press Inc., Totowa NJ 1997

Authors and Affiliations

  • Keith R. Fox
    • 1
  1. 1.Division of Biochemistry and Molecular BiologySchool of Biological Sciences, University of SouthamptonUK

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