Abstract
Nucleic acids labeled with the radioisotope phosphorus-32 [32P] are used as probes for various purposes; coupled with autoradiographic detection, they provide a high degree of sensitivity.
Keywords
- Exonuclease Activity
- Klenow Fragment
- Filter Hybridization
- Nucleic Acid Hybridization
- Tetraethylammonium Chloride
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Rigby, P. W. S., Dieckmann, M., Rhodes, C., and Berg, P. (1977) Labelling deoxy-ribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J. Mol. Biol. 113, 237–251.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Keller, G. H. and Manak, M. M. (1989) DNA Probes. Stockton Press, New York.
Pardue, M. L. (1985) In-situ-hybridisation, in Nucleic Acid Hybridisation: A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, UK, pp. 179–202.
Arrand, J. E. (1985) Preparation of nucleic acid probes, in Nucleic Acid Hybridisation: A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, UK, pp. 17–45.
Henke, L. (1994) Validierung einiger molekularbiologischer Methoden zur Individualisierung und zur Abstammungsbegutachtung des Menschen. Ph.D. dissertation, Univ. Frankfurt/M.
Morris, C. F., Hama-Inaba, H., Mace, D., Sinha, N. K., and Alberts, B. (1979) Purification of the gene 43, 44, 45 and 62 proteins of the bacteriophage T4 DNA replication apparatus. J. Biol. Chem. 254, 6787.
O′Farrell, P. (1981) Replacement synthesis method of labelling DNA fragments. BRLFocus 3, 1.
Feinberg, A. P. and Vogelstein, B. (1983) A technique for radiolabelling DNA restriction endonucleose fragments to high specific activity. Anal. Biochem. 132, 6–13.
Feinberg, A. P and Vogelstein, B. (1984) A technique for radiolabelling DNA restriction endonucleose fragments to high specific activity. Add. Anal. Biochem. 137, 266,267.
Klenow, H., Overgard-Hanson, K., and Patkar, S. (1971) Proteolytic cleavage of native DNA polymerase into two different catalytic fragments. Eur. J. Biochem. 22, 371–381.
Gillespie, D. and Speigelman, S. (1965) A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol. 12, 829.
Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.
Schmitter, H., Berschick, P., Henke, L., and Henke, J. (1995) Molekularbiologische Untersuchungsverfahren in der forensischen Spurenanalyse (DNA-Analysen, DNA-Profiling), in Humanbiologische Spuren (Schleyer, F., Oepen, I., and Henke, J., eds.), Kriminalistik Verlag, Heidelberg, pp. 181–229.
Henke, J., Henke, L., and Cleef, S. (1988) Comparison of different x-ray films for 32P autoradiography using various intensifying screens at −20°C and −70°C. J. Clin. Chem. Clin. Biochem. 26, 467,468.
Anderson, M. L. M. and Young, B. D. (1985) “Quantitative filter hybridisation,” in Nucleic Acid Hybridisation: A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, UK, pp. 73–111.
Young, B. D. and Anderson, M. L. M. (1985) “Quantitative analysis of solution hybridisation in Nucleic Acid Hybridisation: A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, UK, pp. 47–71.
Wetmur, J. G. and Davidson, N. (1968) Kinetics of renaturation of DNA. J. Mol. Biol. 31, 349.
Hutton, J. R. and Wetmur, J. G. (1973) Renaturation of bacteriophage Ø x 174 DNA-RNA hybrid: RNA length effect and nucleation rate constant. J. Mol. Biol. 77, 495.
Bishop, J. O. (1972) Molecular hybridisation of ribonucleic acid with a large excess of deoxyribonucleic acid. Biochem. J. 126, 171.
Studier, F. W. (1969) Effects of the conformation of single stranded DNA on renaturation and aggregation. J. Mol. Biol. 41, 199.
Brittan, R. J., Graham, D. H., and Neufeld, B. R. (1974) in Methods in Enzymology, vol. 29 (Grossmann, L. and Moldave, K., eds.), Academic, London, UK, pp. 363ff.
Chang, C. T., Hain, T. C., Hutton, J. R., and Wetmur, J. G. (1974) Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA. Biopolymers 13, 1847.
Marmur, J. and Doty, P. (1961) Thermal renaturation of deoxyribonucleic acids. J. Mol. Biol. 3, 585.
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Henke, J., Henke, L. (1998). Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity Testing. In: Lincoln, P.J., Thomson, J. (eds) Forensic DNA Profiling Protocols. Methods in Molecular Biology, vol 98. Humana Press. https://doi.org/10.1385/0-89603-443-7:69
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DOI: https://doi.org/10.1385/0-89603-443-7:69
Publisher Name: Humana Press
Print ISBN: 978-0-89603-443-3
Online ISBN: 978-1-59259-204-3
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