Abstract
Hollow-fiber mammalian cell culture systems were first conceived (1) to mimic the in vivo cell environment. In tissues, cells exist immobilized at high density, and are perfused via capillaries having semipermeable walls. Fluid (blood) circulating within the capillaries brings oxygen and nutrients and removes CO2 and other waste products. This description applies equally to hollow-fiber culture systems but with culture medium in the place of blood, and with capillaries made from ultrafiltration or microfiltration membranes.
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© 1997 Humana Press Inc., Totowa, NJ
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Davis, J.M., Hanak, J.A.J. (1997). Hollow-Fiber Cell Culture. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:77
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DOI: https://doi.org/10.1385/0-89603-441-0:77
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-441-9
Online ISBN: 978-1-59259-561-7
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