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Human Pilosebaceous Culture

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Basic Cell Culture Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 75))

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Abstract

The pilosebaceous unit is named after pilus, the Latin for hair, and sebum, the Latin for grease, since it is made of up two components, a hair follicle and a sebaceous gland (Fig. 1). Its culture is important to the study of its diseases, including alopecia and acne; but its culture is also important to the study of skin epithelial stem cells because the epithelial cells of the outer root sheaths of the hair follicle are pluri-potential in their phenotype, and can re-epithelialize the denuded epidermis to heal wounds (1).

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References

  1. Irvin, T. T. (1981) Wound Healing Principles and Practice, Cambridge University Press, Cambridge, UK.

    Google Scholar 

  2. Shapiro, S. S. and Hurley, J. (1990) Effect of retinoids on sebaceous glands. Methods Enzymol. 190, 326–333.

    Article  CAS  PubMed  Google Scholar 

  3. Messenger, A. G. (1984) The culture of dermal papilla cells from human hair follicles. Br. J. Dermatol. 110, 685–689.

    Article  CAS  PubMed  Google Scholar 

  4. Cotsarelis, G., Sun, T.-T., and Lavker, R. M. (1990) Label-retaining cells reside in the bulge area of pilosebaceous units, implications for follicular stem cells, hair cycle and skin carcinogenesis. Cell 61, 1329–1337.

    Article  CAS  PubMed  Google Scholar 

  5. Epstein, E. H. and Epstein, W. L. (1966) New cell formation in human sebaceous glands. J. Invest. Dermatol. 46, 453–458.

    Google Scholar 

  6. Chapman, R. E. (1971) Cell migration in wool follicles of sheep. J. Cell Sci. 9, 791–803.

    CAS  PubMed  Google Scholar 

  7. Stark, H.-J., Breitkreutz, D., Limat, A., Bowden, P., and Fusenig, N. E. (1987) Keratins of the human hair follicle; hyperproliferative keratins are consistently expressed in outer root sheath cells in vivo and in vitro. Differentration 35, 236–248.

    Article  CAS  Google Scholar 

  8. Philpott, M. P., Green, M. R., and Kealey, T. (1990) Human hair growth in vitro. J. Cell Sci. 97, 463–471.

    CAS  PubMed  Google Scholar 

  9. Philpott, M. P. and Kealey, T. (1994) Culture of human pilosebaceous units, in Keratinocyte Methods (Leigh, I. M. and Watt, F. M., eds.), Cambridge University Press, Cambridge, UK, pp. 33–44.

    Google Scholar 

  10. Westgate, G. E., Gibson, W. T., Kealey, T., and Philpott, M. P. (1993) Prolonged growth of human hair follicles in vitro in a serum free defined medium. Br. J. Dermatol. 129, 372–379.

    Article  CAS  PubMed  Google Scholar 

  11. Philpott, M. P., Sanders, S., and Kealey, T. (1995) Cultured human hair follicles and growth factors. J. Invest. Dermatol. 104, 44S,45S.

    Article  CAS  PubMed  Google Scholar 

  12. Williams, D., Profeta, K., and Stenn, K. S. (1994) Isolation and culture of follicular papillae from murine vibrissae an introductory approach. Br. J. Dermatol. 130, 290–297.

    Article  CAS  PubMed  Google Scholar 

  13. Reynolds, A. J. and Jahoda, C. A. B. (1991) Hair follicle stem cells? A distinctive germinative epidermal cell population is activated in vitro by the presence of hair follicle dermal papilla cells. J. Cell Sci. 99, 373–385.

    PubMed  Google Scholar 

  14. Kealey, T. (1990) Effects of retinoids on human sebaceous glands isolated by shearing. Methods Enzymol. 190, 338–346.

    Article  CAS  PubMed  Google Scholar 

  15. Guy, R., Ridden, C., and Kealey, T. (1996) The improved organ maintenance of the human sebaceous gland: modelling in vitro the effects of epidermal growth factor, androgens, oestrogens, 13-cis retinoic acid and phenol red. J. Invest. Dermatol. 106, 454–460.

    Article  CAS  PubMed  Google Scholar 

  16. Leake R. E., Freshney, R. I. and Munir, I. (1987) Steroid response in vivo and in vitro, in Steroid Hormones, a Practical Approach (Green, B. and Leake, R. E., eds.), IRL Press, Oxford, UK, pp. 205–218.

    Google Scholar 

  17. Xia, L., Zouboulis, C., Detmar, M., Mayer-da-Silva, A., Stadler, R., and Orfanos, C. E. (1989) Isolation of human sebaceous glands and cultivation of sebaceous gland-derived cells as an in vitro model. J. Invest. Dermatol. 93, 315–321.

    Article  CAS  PubMed  Google Scholar 

  18. Guy, R., Ridden, C., Barth, J., and Kealey, T. (1993) Isolation and maintenance of the human pilosebaceous duct: 13-cis retinoic acid acts directly on the duct in vitro. Br. J. Dermatol. 128, 242–248.

    Article  CAS  PubMed  Google Scholar 

  19. Guy, R., Green, M. R., and Kealey, T. (1996) Modelling acne in vitro. J. Invest. Dermatol. 106, 176–182.

    Article  CAS  PubMed  Google Scholar 

  20. Hennings, H., Michael, D., Cheng, C., Steinert, P., Holbrook, K., and Yuspa, S. H. (1980) Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 19, 245–254.

    Article  CAS  PubMed  Google Scholar 

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© 1997 Humana Press Inc., Totowa, NJ

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Kealey, T., Philpott, M., Guy, R. (1997). Human Pilosebaceous Culture. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:101

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  • DOI: https://doi.org/10.1385/0-89603-441-0:101

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-441-9

  • Online ISBN: 978-1-59259-561-7

  • eBook Packages: Springer Protocols

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