Abstract
Microorganisms are highly versatile in utilizing a wide variety of compounds as sources of carbon, nitrogen, sulfur, and other nutrients. The catabolic potential of microorganisms can be exploited to destroy undesired pollutants, a process that is now called bioremediatron. The successful application of bioremediation is based on specific knowledge of the chemical structure of the target com- pound(s), the use of approprrate microorganism(s) to attack the pollutant(s), and the operation of the process under relevant conditions, either in situ or ex sztu (it1).
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References
Ramos, J. L. and Trmmts, K. N. (1987) Expertmental evolution of catabolic pathways of bacteria Microbiol Sci 4, 228–237
Kaplan, D. L. (1989) Btotransformatron pathways of hazardous energetic organo-nitro compounds, in Biotechnology and Biodegradation (Kamely, D, Chakrabarty, A, and Omenn, G, ed.), Portfolio Co, Houston, pp. 155–181
Raistrick, H. (1949) Chloromycetm. its structure and synthesis. Nature 163, 5553–5554
Rteger, P. G and Knackmuss, H.J. (1995) Basic knowledge and perspectives on biodegradation of 2,4,6-trmnrotoluene and related nitroaromattc compounds in contaminated soil, in Biodegradation of Nltroaromatzc Compounds (Spain, J. C., ed.), Plenum, New York, pp 1–18
Bruhn, C., Lenke, H., and Knackmuss, H.J. (1987) Nttrosubstituted aromatic compounds as mtrogen source for bacteria Appl. Environ Mlcrobiol. 53, 208–210
Mtchan, C., Delgado, A., Hatdour, A., and Ramos, J L. (1997) In vlvo constructton of a hybrid pathway for the metabolism of 4-mtrotoluene in Pseudomonas fluo-rescens J. Bactenol., submitted for pubhcatron
Duque, E., Haidour, A., Godoy, F, and Ramos, J. L. (1993) Construction of a Pseudomonas hybrid strain that mineralizes 2,4,6-trmttrotoluene J Bactenol. 175, 2278–2283
Ramos, J L., Hardour, A., Delgado, A., Duque, E., Fandtla, M. D., Gil, M., and Piñar, G (1995) Potential of toluene-degradmg systems for the constructton of hybrid pathways for mtrotoluene metabolism, in Biodegradation of Nltroaromatlc Compounds (Spain, J. C., ed), Plenum, New York, pp. 53–68
Crawford, R. (1995) Biodegradation of mtrated munition compounds and herbttides by obltgately anaerobic bacteria, in Bzodegradatton of Nltroaromatlc Compounds (Spain, J. C., ed.). Plenum, New York, pp. 87–98.
Funk, S B., Roberts, D J., Crawford, D L., and Crawford, R L (1993) Imtral-phase opttmtzation for btoremedtatton of mumtton compound-contammated soils Appl. Environ Mlcroblol 59, 2171–2177
Groenewegen, P. E. J., Breeuwer, P., van Helvoort, J. M. L. M., Langenhoff, A. A. M, de Vrtes, F P., and de Bont, J. A. M. (1992) Novel degradattve pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10. J Gen. Microbiol. 138, 1599–1605
Hargler, B. E. and Spain, J C (1991) Biotransformation of mtrobenzene by bacterta containing toluene degradattve pathways. Appl. Environ. Microbiol 57, 3156–3162
Hatgler, B. E. and Spain, J C (1993) Biodegradation of 4-mtrotoluene by Pseudomonas sp. strain 4NT Appl Environ. Mlcrobiol. 59, 2239–2243
Nadeau, L J. and Spain, J C (1995) The bacterial degradation of m-mtrobenzoic acid Appl. Environ Mlcrobiol. 61, 840–843.
Nrshmo, S F. and Spain, J C (1993) Degradatton of mtrobenzene by a Pseudomonas pseudoalcallgenes Appl. Environ. Mlcrobiol 59, 2520–2525.
Rhys-Williams, W., Taylor, S C., and Williams, P A (1993) A novel pathway for catabolism of 4-mtro-toluene bv Pseudomonas J Gen Microbial. 139. 1967–1972
Yamamoto, S and Harayama, S (1995) PCR amphficatton and direct sequencing of gyrB genes with umversal primers and their application to the detection and tax-onomic analysts of Pseudomonas putda strains Appl Environ Microbiol 61, 1104–1109
Durham, N N (1958) Studies on the metabolism of p-mtrobenzoic acid Can J Microbiol 4, 141–148
Ausubel, F M., Brent, R., Kingston, R E., Moore, D D., Smith, J A., Seidman, J G, and Struhl, K. (1987) Current Protocols in Molecular Biology Wiley, New York
Selveral, G and Lyer, V. N (1983) Suicide plasmtd vehicles for insertion mutagen-esis in Rhlzoblum melllotr and related bacterta J Bacteriol 156, 1292–1300
de Lorenzo, V and Timmrs, K N (1994) Analysis and construction of stable phe-notypes in gram-negative bacterta with Tn5 and TnlO-derived mint-transposons Methods Enzymol 235, 386–405
Spain, J C. and Gibson, D T (1991) Pathway for btodegradation of p-mtrophenol in a Moraxella sp Appl Environ Microbiol 57, 812–819.
Jam, R K., Dreisbach, J H, and Spain, J C (1994) Biodegradation of p-mtrophe-no1 via 1,2,4-benzenetriol by an Arthrobacter sp Appl. Envtron Microbiol 60, 3030–3032
Hatgler, B E, Nishmo, S F., and Spain, J C (1994) Biodegradation of 4-methyl-5mtrocatechol by Pseudomonas sp strain DNT J Bactenol 176, 3433–3437
Delgado, A, Wubbolts, M, Abril, M. A., and Ramos, J L (1992) Nttroaromattcs are substrates for the TOL plasmtd upper-pathway enzymes Appl Enwron Microbiol 58, 415–417
Suen, W-C and Spain, J C (1993) Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dmltrotoluene degradation. J. Bacteriol 175, 1831–1837
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© 1997 Humana Press Inc, Totowa, NJ
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Ramos, J.L. et al. (1997). Biodegradation of Nitroaromatics by Microbes. In: Bioremediation Protocols. Methods in Biotechnology™, vol 2. Humana Press. https://doi.org/10.1385/0-89603-437-2:187
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DOI: https://doi.org/10.1385/0-89603-437-2:187
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Print ISBN: 978-0-89603-437-2
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