Abstract
This chapter describes an in vitro system to measure activities of Escherichia coli ribosomes translating poly(U). However, the described techniques are applicable to other translation systems and to other messenger RNAs.
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References
Ehrenberg, M., Bilgm, N., and Kurland, C. G. (1990) Design and use of a fast and accurate in vitro translation system, in Ribosomes and Protein Synthesis A Practical Approach (Spedding, G., ed), IRL Press at Oxford University Press, New York, pp 101–129.
Ruusala, T., Ehrenberg, M., and Kurland, C. G. (1982) Catalytic effects of elongation factor Ts on polypeptide synthesis. EMBO J. 1, 75–78.
Jelenc, P. C. and Kurland, C. G. (1979) Nucleoside triphosphate regeneration decreases the frequency of translation errors. Proc. Natl. Acad. Sci. USA 76, 3174–3178.
Kelmers, A. D., Hamcher, C. W., Phares, E. F., and Novelh, G. D. (1971) Large-scale fermentation of Escherichia coli and recovery of transfer ribonucleic acids, in Methods in Enzymology (Moldave, L., and Grossman, L., eds.), Academic, New York, pp 3–9.
Gillam, I. C. and Tener, G. M. (1971) The use of BD-cellulose in separating transfer RNAs. Meth. Enzymol. 20, 55–70.
Rappoport, S. and Lapidot, Y. (1974) The chemical preparation of acetylamino-acyl-tRNA. Meth. Enzymol. 29, 685–688.
Arai, K., Kawakita, M., and Kaziro, Y. (1974) Studies on the polypeptide elongation factors from E. coli V Properties of various complexes containing EF-Tu and EF-Ts. J. Biochem. (TokyoP 76, 293–306.
Jelenc, P. C. (1980) Rapid purification of highly active ribosomes from Escherichia coli. Analyt. Biochem. 105, 369–374.
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© 1998 Humana Press Inc.
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Bilgin, N., Ehrenberg, M. (1998). Measurement of Rate of Protein Synthesis In Vitro. In: Martin, R. (eds) Protein Synthesis. Methods in Molecular Biology, vol 77. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-397-X:243
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DOI: https://doi.org/10.1385/0-89603-397-X:243
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-397-9
Online ISBN: 978-1-59259-563-1
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