Abstract
The specific binding of lectins to the carbohydrate moieties of glycoproteins and glycohpids on the cell surface has been shown to result in a variety of signal transduction processes (1,2). One of the most dramatic effects of the interaction of lectins with cells is mitogenic stimulation, e.g., the triggering of nondividing lymphocytes into a state of growth and proliferation (3) which has led to the extensive and successful use of lectins in the analysis of events occuring during lymphocyte activation and proliferation (4,5).
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References
Liener, I E, Sharon, N, and Goldstein, I J (1986) The Lectins, Functions, and Applications in Biology and Medicine Academic, New York, pp 270–277
Carraway, K L and Carraway, C A C (1989) Membrane-cytoskeleton interactions in animal cells Biochim Biophys Acta 988, 147–171
Sharon, N (1976) Mitogens in Immunobiology Academic, New York, pp 31–34
Peacock, J S, Colsky, A. S, and Pinto, V. B (1990) Lectins and antibodies as tools for studying cellular interactions J Immunol Methods 126, 147–157
Bates, L G, Grove, D S, and Mastro, A M (1995) Mechanisms of activation and suppression in rat Nb 2 lymphoma cells a model for interactions between prolactin and the immune system Exp Cell Res 218, 567–572
Wright, N. and Alison, M (1984) The Biology of Epithelial Cell Populations Clarendon Press, Oxford, UK, pp 1–20
Feizi, T. (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are oncodevelopmental antigens Nature 314, 53–57
Raz, A and Lotan, R (1987) Endogenous galactoside-binding lectins a new class of functional tumor cell surface molecules related to metastasis Cancer Metasta sis Rev 6, 433–452
Nicolson, G L (1982) Cancer metastasis, organ coloniation and cell-surface properties of malignant cells. Biochim Biophys Acta 95, 113–176
Ryder, S D., Smith, J A, and Rhodes, J. M (1992) Peanut lectin. a mitogen for normal human epithelium and human HT29 colorectal cancer cells. J Natl Cancer Inst 84, 1410–1416
Ryder, S D, Parker, N., Ecclestone, D, Haqqani, M. T, and Rhodes, J M (1994) Peanut lectin stimulates proliferation in colonic explants from patients with inflammatory bowel dizease and colon polyps Gastroenterology 106, 117–124
Yu, L-G, Fernig, D G, Smith, J. A., Milton, J D, and Rhodes, J. M. (1993) Reversible inhibition of proliferatlon of epithelial cell lines by Agaricus bisporus (edible mushroom) lectin Cancer Res 53, 4627–4632
Boulton, R A and Hodgson, H J F (1995) Assessing cell proliferation a methodological review Clin Sci 88, 119–130
Smith, J A, Winslow, D P, and Rudland, P S (1984) Appearance of basic growth factor stimulate cell division of rat mammary epithelial, myoepithelial and stromal cell lines in culture J Cell Physiol 119, 320–328
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© 1998 Humana Press Inc.
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Yu, LG., Rhodes, J.M. (1998). Mitogenic Effects of Lectins on Epithelial Cells. In: Rhodes, J.M., Milton, J.D. (eds) Lectin Methods and Protocols. Methods in Molecular Medicine™, vol 9. Humana Press. https://doi.org/10.1385/0-89603-396-1:379
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DOI: https://doi.org/10.1385/0-89603-396-1:379
Publisher Name: Humana Press
Print ISBN: 978-0-89603-396-2
Online ISBN: 978-1-59259-593-8
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