Abstract
Microdialysis is a frequently used technique to collect continuously in vivo various endogenous chemical substances from the extracellular space of discrete brain regions. In comparison to other sampling methods (such as the push-pull technique), microdialysis causes only minor tissue trauma. This feature makes microdialysis the technique of choice when studying in vivo extracellular transmitter levels in relationship to diverse physiological and pathophysiological processes in several species, including humans (1,2). Because of limitations of the technique, microdialysis methods are unsuitable for analysts of rapid, transient changes in extracellular transmitter levels. The microdialysis technique is, however, well suited to detect slow alterations in neurotransmitter concentrations, as occurring, for example, during ischemia (3).
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Ungerstedt, U. (1991) Introduction to intracerebral microdialysis, in: Microdialysis in the Neurosciences (Robinson, T. E. and Justice, J. B., Jr., eds.), Elsevier Science, B. V., Amsterdam, pp. 2–22.
During, M. J., Ryder, K. M., and Spencer, D. D. (1995) Hippocampal GABA transporter function in temporal-lobe epilepsy. Nature 376, 174–177.
Globus, M. Y.-T., Busto, R., Martinez, E., Valdes, I., Dietrich, W. D., and Ginsberg, M. D. (1991) Comparative effect of transient global ischemia on extracellular levels of glutamate, glycine and γ-aminobutyric acid in vulnerable and non-vulnerable brain regions in the rat. J. Neurochem. 57, 470–478.
Westerink, B. H. C., Damsma, G., Rollema, H., De Vries, J. B., and Horn, A. S. (1987) Scope and limitations of in vivo brain dialysis: a comparison of its application to various neurotransmitter systems. Life Sci. 41, 1763–1776.
Attwell, D. and Mobbs, P. (1994) Neurotransmitter transporters. C. Op Neurobiol, 4 353–359.
Bliss, T. V. P. and Collinridge, G. L. (1993) A synaptic model of memory: long-term potentiation in the hippocampus. Nature 361, 31–39.
Kamphuis, W. and Lopes da Silva, F. H. (1990) The kindling model of epilepsy. the role of GABAergic inhibition. Neurosci. Res. Comm. 6, 1–10.
Zuiderwijk, M., Veenstra, E., Lopes da Silva, F. H., and Ghijsen, W. E. J. M. (1996) Effects of uptake carrier blockers SK&F 89976-A and L-trans-PDC on in vivo release of amino acids in rat hippocampus. Eur. J. Pharmacol. 307, 275–282.
Pellegrino, L. K., Pellegrino, A. S., and Cushman, A. J. (1979) A Stereotaxic Atlas of the Rat Brain. Plenum, New York, NY.
Benveniste, H. and Huttemeier, P. C. (1990) Microdialysis—theory and application. Prog Neurobiol. 35, 195–215
Verhage, M., Besselsen, E., Lopes da Silva, F. H., and Ghijsen, W. E. J. M. (1989) Ca2+-dependent regulation of presynaptic stimulus-secretion coupling. J Neurochem. 53, 1188–1194.
Griffiths, R., Dunlop, J., Gorman, A., Senior, J., and Grieve, A. (1994) L-trans-pyrrolidine-2,4,-dicarboxylates behave as transportable, competitive inhibitors of the high-affinity glutamate transporters. Biochem Pharmacol 4, 267–274.
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© 1997 Humana Press Inc., Totowa, NJ
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Zuiderwijk, M., Ghijsen, W.E.J.M. (1997). Monitoring Amino Acid Neurotransmitter Release in the Brain by In Vivo Microdialysis. In: Rayne, R.C. (eds) Neurotransmitter Methods. Methods in Molecular Biology, vol 72. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-394-5:239
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DOI: https://doi.org/10.1385/0-89603-394-5:239
Publisher Name: Springer, Totowa, NJ
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