Skip to main content

Carlavirus Isolation and RNA Extraction

  • Protocol
Plant Virology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 81))

Abstract

All members of the genus are known to be transmitted mechanically, with the majority also being transmitted in a nonpersistent manner by aphids (1), though one confirmed carlavirus is known to be transmitted by whiteflies (2). Carlaviruses are noted for their narrow host range and tendency to induce little or no symptoms. This has led to many of the common names of carlaviruses, including carnation latent (CLV), American hop latent (AHLV), and lily symptomless virus (LSV). Although most carlaviruses do cause mild symptoms, there are a number of viruses that cause serious diseases on their own, for example, potato virus S (PVS) and M (PVM), blueberry scorch virus (BBScV), poplar mosaic virus (PMV), and a number of others that cause serious disease in mixed viral infections (with other viruses).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Foster, G. D. (1992) The structure and expression of the genome of carlaviruses. Res. Virol. 143, 103–112.

    Article  PubMed  CAS  Google Scholar 

  2. Badge, J., Brunt, A., Carson, R., Dagless, E., Karamagioli, M., Phillips, S., Seal, S., Turner, R., and Foster, G. D. (1996) A carlavirus-specific PCR primer and partial nucleotide sequence provides further evidence for the recognition of cowpea mild mottle virus as a whitefly-transmitted carlavirus. Eur. J. Plant Pathol. 102, 305–310.

    Article  CAS  Google Scholar 

  3. Brunt, A. A. (1995) Carlaviruses, in Virus Taxonomy: Sixth Report of the International Committee on Taxonomy of Viruses (Murphy, F. A., Fauquet, C. M., Bishop, H. L., Ghabrial, S. A., Jarvis, A. E., Martelli, G. P., Mayo, M. A., and Summers, M. D., eds.), Springer-Verlag, Wien, New York.

    Google Scholar 

  4. Zavriev, S. K., Kanyuka, K. V., and Leavy, K. E. (1991) The genome organisation of potato virusM RNA. J. Gen. Virol. 72, 9–14.

    Article  PubMed  CAS  Google Scholar 

  5. Cavileer, T. D., Halpern, B. T., Lawrence, D. M., Podleckis, E. V., Martin, R. R., and Hillman, B. I. (1994) Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. J. Gen. Virol. 75, 711–720.

    Article  PubMed  CAS  Google Scholar 

  6. Foster, G. D. and Mills, P. R. (1990) Evidence for the role of subgenomic RNA species in the production of Helenium virus S coat protein during in vitro translation. Virus Res. 17, 61–70.

    Article  CAS  Google Scholar 

  7. Foster, G. D. and Mills, P. R. (1992) Translation of potato virus S RNA in vitro: evidence of protein processing. Virus Genes 6, 47–52.

    Article  PubMed  CAS  Google Scholar 

  8. Lawrence, D. M., Rozanov, M. N., and Hillman, B. I. (1995) Autocatalytic processing of the 223-kDa protein of blueberry scorch carlavirus by a papain-like protein-ase. Virology 207, 127–135.

    Article  PubMed  CAS  Google Scholar 

  9. Foster, G. D. and Mills, P. R. (1991) Evidence for subgenomic RNAs in leaves infected with an Andean strain of potato virus S. Acta Virologica 35, 260–267.

    PubMed  CAS  Google Scholar 

  10. Foster, G. D. and Mills, P. R. (1990) Evidence for the role of subgenomic RNAs in the production of potato virus S coat protein during in vitro translation. J. Gen. Virol. 71, 1247–1249.

    Article  PubMed  CAS  Google Scholar 

  11. Shields, S. A. and Wilson, T. M. A. (1987) Cell free translation of turnip mosaic virus RNA. J. Gen. Virol. 68, 169–180.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc.

About this protocol

Cite this protocol

Foster, G.D. (1998). Carlavirus Isolation and RNA Extraction. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:145

Download citation

  • DOI: https://doi.org/10.1385/0-89603-385-6:145

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-385-6

  • Online ISBN: 978-1-59259-566-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics