Abstract
The Lambda ZAP® vectors have been designed to allow in vivo excision and recircularization of the cloned insert and phagemid sequences contained within the λ vector (1). Once a Lambda ZAP library is constructed and amplified, putative clones or the λ library itself may be excised into the phagemid form. Two versions of the excision protocol for Lambda ZAP-based vectors are included here.
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References
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© 1997 Humana Press Inc., Totowa, NJ
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Snead, M.A., Alting-Mees, M.A., Short, J.M. (1997). Clone Excision Methods for the Lambda ZAP®-Based Vectors. In: Cowell, I.G., Austin, C.A. (eds) cDNA Library Protocols. Methods in Molecular Biology™, vol 69. Humana Press. https://doi.org/10.1385/0-89603-383-X:53
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DOI: https://doi.org/10.1385/0-89603-383-X:53
Publisher Name: Humana Press
Print ISBN: 978-0-89603-383-2
Online ISBN: 978-1-59259-555-6
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