Expression and Preparation of Fusion Proteins from Recombinant λgt1.1 Phages

Abstract

The phage λgt1.1 system has become increasingly popular for expression of cDNAs or genomic DNAs either in phage plaques or in bacteria lysogenized with recombinant phages (1,2). It offers the advantages of high cloning efficiency, high-level expression, the relative stability of β-galactosidase fusion proteins, and simple approaches to purify the fusion proteins. After the desired clone is detected and purified, it is often necessary to obtain preparative amounts of recombinant protein specified by the fusion of the foreign sequence to the carboxyl-terminus of β-galactosidase in λgt1.1 expression system. The conventional method for preparing fusion proteins from the recombinant λgt1.1 clones involves production of phage lysogens in Escherichia coli strain Y1089 followed by inducing lacZ-directed fusion protein expression with isopropyl-β-D-thiogalactopyranoside (IPTG) (1). This method has two limitations: it is time-consuming, and phage lysogeny occurs at a low frequency. We have previously described a method for making fusion proteins from LB agar plates containing E. coli Y1090 infected with a high concentration of recombinant λgt1.1 phages (up to 5 × 10⁶ PFU/l50 × 15-mm plate) (3). A liquid culture method for preparing fusion proteins from E. coli. Y1090 infected with the λgt1.1 clones has previously been described (4). More recently, some improvements have been made on the plate method by repeating induction and elution (5). Although the liquid culture method allows the recovery of only 0.2–1% of total proteins (6,7), this method generally yields 5–10% of expressed protein in solution, that is, most lysed cells are trapped in the agar and the expressed proteins are recovered in a small volume of inducing solution, resulting in a higher final concentration of protein. More than 200 µg of fusion protein can be obtained from one plate. Currently, the plate method appears to be a simple and efficient way to express and prepare fusion proteins from recombinant λgt1.1 phages (Figs. 1 and 2). This chapter describes how the β-galactosidase fusion proteins can be made and isolated from the recombinant λgt1.1 phages with the plate method.