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Isolation and Characterization of Porins from H. pylori

  • Protocol
Book cover Helicobacter pylori Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 8))

Abstract

The outer membranes of Gram-negative bacteria represent selective, permeability barriers to environmental molecules. This function is accomplished in two ways. First, outer membranes exclude many larger hydrophilic molecules, including enzymes and other proteins, and most hydrophobic ones, by virtue of their unique composition. It has been demonstrated in Escherichia coli that outer membranes are asymmetric bilayers containing a unique species of glycolipid, lipopolysaccharide (LPS), in its outer leaflet, and phospholipids in its inner leaflet. The highly negatively charged LPS is stabilized by divalent cations. The observations that Helicobacter outer membrane proteins are Triton X-100 insoluble in the absence of EDTA or NaCl (1,2), that Helicobacter contains substantial amounts of LPS 3, and that Helicobacter is resistant to highly hydrophobic antibiotics, such as trimethoprin and nalidixic acid (4), lead one to believe that this organism is thematically similar.

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Author information

Authors and Affiliations

  1. Canadian Bacterial Diseases Network and Department of Microbiology, University of British Columbia, Vancouver, British Columbia, Canada

    Robert E. W. Hancock & Maurice Exner

Authors
  1. Robert E. W. Hancock
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  2. Maurice Exner
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Editor information

Editors and Affiliations

  1. Glaxo Wellcome Research and Development Ltd., Stevenage, UK

    Christopher L. Clayton

  2. University of Maryland School of Medicine, Baltimore, MD

    Harry L. T. Mobley

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© 1997 Humana Press Inc, Totowa, NJ

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Hancock, R.E.W., Exner, M. (1997). Isolation and Characterization of Porins from H. pylori . In: Clayton, C.L., Mobley, H.L.T. (eds) Helicobacter pylori Protocols. Methods in Molecular Medicine, vol 8. Humana Press. https://doi.org/10.1385/0-89603-381-3:191

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  • DOI: https://doi.org/10.1385/0-89603-381-3:191

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-381-8

  • Online ISBN: 978-1-59259-592-1

  • eBook Packages: Springer Protocols

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