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Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 cells

  • Protocol
HIV Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 17))

Abstract

HIV-1 is routinely isolated by cocultivation of patient PBMC with mitogen-stimulated HIV-uninfected donor PBMC (see Chapter 1). In this culture system, HIV-1 primarily replicates in CD4+ T-lymphocytes, and such viruses are termed clinical or primary isolates. As early as 1986, the in vitro replicative capacity and cell tropism of primary HIV-1 isolates were shown to be important in the pathophysiology of HIV-1 infection (1). High replication capacity in PBMC and virus growth and syncytium formation in neoplastic T-cell lines were found to correlate with severity of HIV-1-disease (2–5). Compared to syncytium-inducing (SI) isolates, nonsyncytium-inducing (NSI) strains did not replicate in neoplastic T-cell lines and showed preferential replication in cells of the monocyte-macrophage lineage (6,7). Thus, NSI viruses have often been termed macrophage tropic, whereas SI strains are termed T-cell line tropic.

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Author information

Authors and Affiliations

  1. Henry M. Jackson Foundation, Rockville, MD

    Mark K. Louder

  2. Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD

    John R. Mascola

Authors
  1. Mark K. Louder
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  2. John R. Mascola
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Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Rockville, MD

    Nelson L. Michael MD, PhD

  2. Henry M. Jackson Foundation, Rockville, MD

    Jerome H. Kim MD

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© 1999 Humana Press Inc., Totowa, NJ

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Louder, M.K., Mascola, J.R. (1999). Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 cells. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:23

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  • DOI: https://doi.org/10.1385/0-89603-369-4:23

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-369-6

  • Online ISBN: 978-1-59259-601-0

  • eBook Packages: Springer Protocols

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