Abstract
The CCR5 gene encodes a cell-surface chemokine receptor molecule, which serves as a coreceptor for macrophage-tropic strains of HIV-1 (1–3). Mutations in this gene may alter expression or function of the protein product, thereby altering chemokine binding or HIV-1 infection of cells on which the receptor is normally expressed. Indeed, it was recently shown that individuals homozygous for a mutant allele (CCR5-△32) characterized by a 32-bp deletion in the coding region of the CCR5 gene, are relatively resistant to HIV-1 infection (4–6). This allele causes a frame shift at amino acid 185, and homozygous individuals fail to express detectable cell-surface CCR5 molecules. It is possible that other as-yet unidentified variants play a role in HIV-1 infectivity and outcome. Several additional mutations, most of which are single-base substitutions, have been identified in the coding region of the CCR5 gene using the single-strand conformation polymorphism (SSCP) technique (7).
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© 1999 Humana Press Inc., Totowa, NJ
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Martin, M.P., Carrington, M. (1999). Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:205
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DOI: https://doi.org/10.1385/0-89603-369-4:205
Publisher Name: Humana Press
Print ISBN: 978-0-89603-369-6
Online ISBN: 978-1-59259-601-0
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