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Molecular Diagnosis of Cancer pp 141–147Cite as

Mutational Analysis of the Wilms' Tumor (WTI) Gene

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 6))

Abstract

Mutations of the Wilms’ tumor (WT1) gene have been shown to underlie a proportion of cases of Wilms’ tumor, an embryonal kidney cancer occurring mainly in childhood. The WTl gene comprtses ten exons spanning approx 50 kb of genomrc DNA. The messenger RNA is approx 3 kb in length and encodes a zinc finger protein. The four zinc fingers, which he at the C-terminal end of the protein, are encoded by separate exons 7–10. The 5′ end of the gene is extremely GC-rich, with areas approaching a 70% GC content. This makes this region difficult to amplify in polymerase chain reactions.

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Authors and Affiliations

  1. Section of Paediatrics, Institute of Cancer Research, Sutton, UK

    Linda King-Underwood & Kathy Pritchard-Jones

Authors
  1. Linda King-Underwood
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  2. Kathy Pritchard-Jones
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Editors and Affiliations

  1. University London, UK

    Finbarr E. Cotter

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© 1996 Humana Press Inc., Totowa, NJ

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King-Underwood, L., Pritchard-Jones, K. (1996). Mutational Analysis of the Wilms' Tumor (WTI) Gene. In: Cotter, F.E. (eds) Molecular Diagnosis of Cancer. Methods in Molecular Medicine™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-341-4:141

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  • DOI: https://doi.org/10.1385/0-89603-341-4:141

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-341-2

  • Online ISBN: 978-1-59259-590-7

  • eBook Packages: Springer Protocols

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